Mui K, Yamagami S, Kioka T, Onishi H, Kawakita Y
Department of Neuropsychiatry, Osaka City University Medical School, Japan.
Epilepsy Res. 1991 Nov-Dec;10(2-3):134-41. doi: 10.1016/0920-1211(91)90005-z.
The hybridization kinetics in the complementary DNAs (cDNA) to polyadenylated messenger RNA (mRNA) in the brain of seizure-susceptible El mice were examined to elucidate the correlation between seizure susceptibility and genetic expression. Homologous and heterologous saturation hybridizations indicated that cDNA of seizure-nonsusceptible ddY mice hybridized with less mRNA in seizure-experienced El(+) or seizure-nonexperienced El(o) mice than that in ddY mice, cDNA of El(+) mice hybridized to a lesser extent with mRNA of El(o) mice than that of ddY or El(+) mice, and no difference was observed with cDNA hybridization results of El(o) mice to individual mRNAs of ddY, El(+) or El(o) mice. The results suggest that some sequences found in mRNA of ddY are lacking in both El strains and are responsible for the inbred predisposition, while those present in El(+) mRNA but missing in El(o) may associate with seizure susceptibility, since common sequences are present in the mRNA of those groups of animal. The sequence diversity among the 3 groups of mice was mainly observed with the rare class of mRNA. Molecular cloning of the cDNA corresponding to this class of mRNA found in the El(+) mice would clarify the gene expression in the brains of epileptics.
研究了癫痫易感El小鼠大脑中互补DNA（cDNA）与多聚腺苷酸化信使RNA（mRNA）的杂交动力学，以阐明癫痫易感性与基因表达之间的相关性。同源和异源饱和杂交表明，癫痫非易感ddY小鼠的cDNA与经历过癫痫发作的El(+)或未经历过癫痫发作的El(o)小鼠的mRNA杂交程度低于与ddY小鼠mRNA的杂交程度，El(+)小鼠的cDNA与El(o)小鼠mRNA的杂交程度低于与ddY或El(+)小鼠mRNA的杂交程度，并且未观察到El(o)小鼠的cDNA与ddY、El(+)或El(o)小鼠的单个mRNA杂交结果存在差异。结果表明，ddY小鼠mRNA中发现的一些序列在两种El品系中均不存在，并且与近交易感性有关，而El(+) mRNA中存在但El(o)中缺失的那些序列可能与癫痫易感性有关，因为这些动物群体的mRNA中存在共同序列。三组小鼠之间的序列多样性主要在稀有类别的mRNA中观察到。对在El(+)小鼠中发现的这类mRNA对应的cDNA进行分子克隆，将阐明癫痫患者大脑中的基因表达情况。
