Han H, Shao D, Wu J, Cornélissen G, Halberg F
Institute of Biophysics, Academia Sinica, Beijing, China.
Cell Biophys. 1991 Jun;18(3):217-29. doi: 10.1007/BF02989815.
An earlier demonstration of a circadian rhythm in rat atria by others is complemented herein by observations in culture: A single murine myocardial cell and two sets of grouped cells beating in culture for several days reveal several features of an anticipated, presumably built-in spectrum of multifrequency rhythms and trends, the chronome. Circadian and about 12-h (circasemidian) components are modulated by an approximately 84-h (circasemiseptan) component, which cannot be separated from trends in view of the brevity of the series. The circumstance under which the culture is aging and in which fibroblasts proliferate is a further complication that limits the findings to a single cycle reproduced in three separate cultures. Whether it is a rhythm that repeats itself of a response to placement into culture, an approximately 3.5-d component in the beating of myocardial cells in culture is to be aligned with a very prominent similar component found in the incidence of 85,819 human myocardial infarctions.
其他人早期对大鼠心房昼夜节律的证明，在此通过培养实验的观察得到补充：单个小鼠心肌细胞以及两组在培养中搏动数天的成组细胞，揭示了预期的、可能内在的多频节律和趋势谱（生物钟）的几个特征。昼夜节律和大约12小时（近半日）的成分受大约84小时（近一周）的成分调节，鉴于实验系列的简短，该成分无法与趋势分开。培养物老化且成纤维细胞增殖的情况是另一个复杂因素，这将研究结果限制在三个独立培养物中重现的单个周期。无论是自身重复的节律还是对培养环境的反应，培养中心肌细胞搏动中大约3.5天的成分，都与85819例人类心肌梗死发生率中发现的一个非常显著的类似成分一致。
