使用含硫代磷酸酯mRNA的无细胞翻译系统。

Cell-free translation system using phosphorothioate-containing mRNA.

作者信息

Ueda T, Tohda H, Chikazumi N, Eckstein F, Watanabe K

机构信息

Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Nucleic Acids Symp Ser. 1991(25):151-2.

PMID:1726804

Abstract

Phosphorothioate-containing RNAs were generated by transcription of template DNA using the Sp diastereomers of ribonucleoside 5'-O-(1-thiotriphosphates) (NTP alpha S) and T7 RNA polymerase. The substitution of mRNA by phosphorothioate increased the efficiency of protein synthesis by stabilizing the mRNAs in prokaryotic cell-free translation systems. The substituted mRNAs were also shown to be applicable to the continuous cell-free translation system developed by Spirin and coworkers.

摘要

含硫代磷酸酯的RNA是通过使用核糖核苷5'-O-(1-硫代三磷酸酯)(NTPαS)的Sp非对映异构体和T7 RNA聚合酶转录模板DNA生成的。硫代磷酸酯取代mRNA通过在原核无细胞翻译系统中稳定mRNA提高了蛋白质合成效率。取代的mRNA也被证明适用于斯皮林及其同事开发的连续无细胞翻译系统。