Hirao I, Yoshizawa S, Miura K
Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
Nucleic Acids Symp Ser. 1990(22):75-6.
In the RNA directed cell-free protein synthesizing system from E. coli, there is a problem of contaminating 3'-exonucleases which attack the mRNAs. Thus, we tried the following two methods to stabilize mRNAs against nucleases: (A) To use mRNAs having hairpin structures at their termini and (B) To hybridize mRNAs with small DNA fragments to the 3'-termini of mRNAs. It was found that degradation of a mRNA was inhibited by the method B rather than the method A in the translation system.
在来自大肠杆菌的RNA定向无细胞蛋白质合成系统中，存在3'-核酸外切酶污染的问题，这些酶会攻击mRNA。因此，我们尝试了以下两种方法来稳定mRNA以抵抗核酸酶：（A）使用在其末端具有发夹结构的mRNA，以及（B）将mRNA与小DNA片段杂交至mRNA的3'-末端。发现在翻译系统中，方法B比方法A更能抑制mRNA的降解。
