Hong Yu, Wang Lin-He, Zhang Guosheng, Enhe Bayaer, Liang Xiaorong
College of Ecology and Environment, Inner Mongolia Agricultural University, Huhhot 010022, China.
Ying Yong Sheng Tai Xue Bao. 2006 Nov;17(11):2006-10.
By means of random amplified polymorphic DNA markers, a molecular ecological study was made with Sabina vulgaris populations at 4 succession stages in Maowusu sandy grassland, aimed to reveal the relationships between molecular variation and succession stages. A total of 17 random primers were selected for amplification, and 190 repetitive loci were produced, of which, 173 were polymorphic. The data were analyzed by POPGENE 3. 2 Version 1. 31. The results showed that the genetic diversity of S. vulgaris populations was high, and changed with succession stages. The percentage of polymorphic loci in each S. vulgaris population ranged from 64.21% to 74.63%, with the highest in early succession stage Artemisia ordosica + S. vulgaris on semi-fixed sand dunes, and the lowest in sub-climax stage S. vulgaris on fixed dunes. The genetic differentiation among the populations was small (G(st) = 0.1761), and 82.39% of it was within the populations. Cluster analysis demonstrated that the populations at similar succession stage clustered together, suggesting that the genetic differentiation was closely related to succession stage. The genetic diversity indicated by Nei index ranged in 0. 2163 -0. 2564, and the gene flow (N(m) *) was 2.7972, indicating that more gene exchange occurred within the populations, which prevented the genetic differentiation among the populations at different succession stages.
采用随机扩增多态性DNA标记,对毛乌素沙地草地4个演替阶段的沙地柏种群进行了分子生态学研究,旨在揭示分子变异与演替阶段之间的关系。共选择17条随机引物进行扩增,产生了190个重复位点,其中173个具有多态性。利用POPGENE 3.2版本1.31对数据进行分析。结果表明,沙地柏种群的遗传多样性较高,且随演替阶段而变化。各沙地柏种群的多态位点百分比在64.21%至74.63%之间,在半固定沙丘上的演替早期阶段油蒿+沙地柏种群中最高,在固定沙丘上的亚顶极阶段沙地柏种群中最低。种群间的遗传分化较小(G(st)=0.1761),其中82.39%的变异存在于种群内。聚类分析表明,处于相似演替阶段的种群聚在一起,表明遗传分化与演替阶段密切相关。Nei指数表示的遗传多样性在0.2163 - 0.2564之间,基因流(N(m)*)为2.7972,表明种群内发生了更多的基因交换,这阻止了不同演替阶段种群间的遗传分化。