Mo Ai-Qiong, Geng Shi-Lei, Zhang Shou-Zhou
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Zhong Yao Cai. 2009 Apr;32(4):485-90.
To analyze the genetic diversity and differentiation degree of five Lonicera confusa populations.
Random Amplified Polymorphic DNA (RAPD) markers were perfomed to amplify the discernible loci.
A total of 100 loci were obtained for all populations using ten primers, 87.00% of which were polymorphic (PPL = 87.00%). Analyzed by POPGENE, the average percentage of polymorphic loci was 58.86%. Nei's genetic diversity (H = 0.2647) and Shannon's information index (I = 0.4022) indicated that Lonicera confusa had a higher level of genetic diversity. The high value of differentiation( G(st) = 0.3839) showed that geographic isolation strongly influenced genetic differentiation among populations and there was a significant correlation between genetic distance and geographic distance (r = 0.8504, P = 96.80%). The clustering analysis was performed using softwares POPGENE and NTSYS-pc. Five populations were divided into 2 clusters, named cluster 1 (LFS, XX and NN) and cluster 2 (XW and HK).
Management strategies were proposed for species conservation and resource utilization of Lonicera confusa, including the protection from human disturbance to facilitate natural regeneration, construction of germplasm resources and cultivation of plants collected from different populations, in order to select elite lines for good agricultural practice (GAP) of medicinal materials.
分析五个山银花种群的遗传多样性及分化程度。
采用随机扩增多态性DNA（RAPD）标记对可识别位点进行扩增。
使用10条引物对所有种群共获得100个位点，其中87.00%为多态性位点（PPL = 87.00%）。经POPGENE分析，多态性位点的平均百分比为58.86%。Nei氏遗传多样性（H = 0.2647）和Shannon信息指数（I = 0.4022）表明山银花具有较高水平的遗传多样性。分化系数较高（Gst = 0.3839），表明地理隔离对种群间的遗传分化有强烈影响，且遗传距离与地理距离之间存在显著相关性（r = 0.8504，P = 96.80%）。使用POPGENE和NTSYS-pc软件进行聚类分析。五个种群被分为2个类群，命名为类群1（LFS、XX和NN）和类群2（XW和HK）。
提出了山银花物种保护和资源利用的管理策略，包括防止人为干扰以促进自然更新、构建种质资源以及种植从不同种群收集的植物，以便选择优良品系用于药材的良好农业规范（GAP）。
