Sachl Radek, Uchman Mariusz, Matĕjícek Pavel, Procházka Karel, Stĕpánek Miroslav, Spírková Milena
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Albertov 2030, 128 40 Prague 2, Czech Republic.
Langmuir. 2007 Mar 13;23(6):3395-400. doi: 10.1021/la063014c. Epub 2007 Feb 2.
Aqueous solutions of self-assembled nanoparticles formed by biocompatible diblock copolymers of poly(epsilon-caprolactone)-block-poly(ethylene oxide) (PCL-PEO) with the same molar mass of the PEO block (5000 g mol-1) and three different molar masses of the PCL block (5000, 13 000, and 32 000 g mol-1) have been prepared by a fast mixing the copolymer solution in a mild selective solvent, tetrahydrofuran (THF)/water, with an excess of water, that is, by quenching the reversible micellization equilibrium, and a subsequent removal of THF by dialysis of the water-rich solution against water. The prepared nanoparticles have been characterized by static and dynamic light scattering and atomic force microscopy imaging. It was found that stable monodisperse nanoparticles are formed only if the initial mixed solvent contained 90 vol % THF. The results show that the prepared nanoparticles are spherical vesicles with relatively thick hydrophobic walls, that is, spherical core/shell nanoparticles with the hollow core filled with the solvent.
通过将聚（ε-己内酯）-嵌段-聚（环氧乙烷）（PCL-PEO）的生物相容性二嵌段共聚物在温和的选择性溶剂四氢呋喃（THF）/水中的溶液与过量的水快速混合来制备纳米颗粒的水溶液，也就是说，通过猝灭可逆胶束化平衡，然后通过将富水溶液对水进行透析来除去THF。所制备的纳米颗粒已通过静态和动态光散射以及原子力显微镜成像进行了表征。发现仅当初始混合溶剂含有90体积％的THF时才形成稳定的单分散纳米颗粒。结果表明，所制备的纳米颗粒是具有相对较厚疏水壁的球形囊泡，即具有填充有溶剂的中空核的球形核/壳纳米颗粒。
