Stein L S, Boonstra J, Burghardt R C
Department of Veterinary Anatomy, Texas A&M University, College Station 77843.
Exp Cell Res. 1992 Jan;198(1):1-7. doi: 10.1016/0014-4827(92)90141-t.
The effects of mitosis on gap junctional intercellular communication (GJIC) were quantified in a clonal cell line of spontaneously immortalized rat granulosa cells (SIGC) using a fluorescence recovery after photobleaching assay. Reduction of GJIC was associated with the process of mitosis and was first apparent at the onset of prophase. Resumption of GJIC between newly divided cells and surrounding cells occurred slowly, requiring several hours following cytokinesis before reestablishment of maximal rates. Mitotic rates of GJIC in SIGC were comparable to values obtained in interphase cells partially uncoupled by 0.5 mM octanol. Limited studies of other cell lines generalized the mitotic-associated reduction of GJIC observed in SIGC. The data suggest that mitosis is one process which alters GJIC. This could be of significance when there is a change in the rate of proliferation, such as in the acquisition of immortalization, an early stage of transformation.
利用光漂白后荧光恢复试验，在自发永生化大鼠颗粒细胞（SIGC）的克隆细胞系中对有丝分裂对间隙连接细胞间通讯（GJIC）的影响进行了定量分析。GJIC的减少与有丝分裂过程相关，在前期开始时首次明显出现。新分裂细胞与周围细胞之间GJIC的恢复过程缓慢，在胞质分裂后需要数小时才能重新建立最大速率。SIGC中有丝分裂时GJIC的速率与用0.5 mM辛醇部分解偶联的间期细胞中获得的值相当。对其他细胞系的有限研究推广了在SIGC中观察到的有丝分裂相关的GJIC减少现象。数据表明，有丝分裂是改变GJIC的一个过程。当增殖速率发生变化时，如在永生化获得（转化的早期阶段）过程中，这可能具有重要意义。
