陡脉冲电场对荷瘤BALB/c小鼠的致死和抑制作用

Lethal and inhibitory effects of steep pulsed electric field on tumor-bearing BALB/c mice.

作者信息

Mi Yan, Sun Caixin, Yao Chenguo, Xiong Lan, Liao Ruijin, Hu Ya, Hu Lina

机构信息

Key Lab of High Voltage Engineering and Electrical New Technology, Ministry of Education, Chongqing University, P. R. China.

出版信息

Conf Proc IEEE Eng Med Biol Soc. 2004;2004:5005-8. doi: 10.1109/IEMBS.2004.1404383.

DOI:10.1109/IEMBS.2004.1404383

PMID:17271439

Abstract

Exposing cancer cell to steep pulsed electric field (SPEF) with appropriate dosage, the cell may die due to irreversible electrical breakdown (IREB). 48 BALB/c mice inoculated with U/sub 14/ cervical cancer cell were treated with SPEF to study the lethal and inhibitory effects of SPEF on them. They were randomly divided into test group (26) and control group (22), and then four experiments were carried out. Histological observation and ultrastructural observation showed that SPEF destroyed the integrality of tumor cell irreversibly with pyknosis, karyoclasis and karyolysis. Tumor volume measurement indicated that SPEF significantly slowed down the growth speed of tumor. In the experiment of survival time investigation, the average survival time of control group and test group was 33.03 days and no less than 52.05 days respectively, which confirmed that SPEF prolonged the survival time of tumor-bearing mice obviously. With further experimental study and mechanism analysis, this technique is quite possible to be an effective physiotherapy for tumor treatment.

摘要

将癌细胞暴露于适当剂量的陡脉冲电场（SPEF）下，细胞可能会因不可逆电击穿（IREB）而死亡。对接种U/sub 14/宫颈癌细胞的48只BALB/c小鼠进行了SPEF处理，以研究SPEF对它们的致死和抑制作用。将它们随机分为试验组（26只）和对照组（22只），然后进行了四项实验。组织学观察和超微结构观察表明，SPEF通过核固缩、核碎裂和核溶解不可逆地破坏了肿瘤细胞的完整性。肿瘤体积测量表明，SPEF显著减缓了肿瘤的生长速度。在生存时间调查实验中，对照组和试验组的平均生存时间分别为33.03天和不少于52.05天，这证实了SPEF明显延长了荷瘤小鼠的生存时间。随着进一步的实验研究和机制分析，该技术很有可能成为一种有效的肿瘤物理治疗方法。