Masaki Hiroyuki, Korenaga Takashi
Department of Chemistry, Graduated School of Science, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji Tokyo 192-0397, Japan.
Environ Sci. 2006;13(6):353-62.
We developed a specific analysis method for benzo[a]pyrene (B[a]P) and benzo[k]fluoranthene (B[k]F) using dual beam fluorometry. B[a]P and B[k]F exhibited two specific fluorescence intensities (up to 10-fold) compared with 22 other polycyclic aromatic hydrocarbons (PAHs) at 370 nm excitation and 429 nm emission wavelengths, and similarly at 395 nm excitation and 429 nm emission wavelengths. The concentrations of these compounds in diesel exhaust particles were calculated from two calibration curves determined using a mixture of 24 PAH standards. The precision of this detection method was compared with that of conventional high-performance liquid chromatography/fluorescene detector (HPLC/FLD), and our method gave accurate values within the analytical standard deviations. The monitoring method developed in this study will enable more rapid and easier detection.
我们开发了一种使用双光束荧光法分析苯并[a]芘（B[a]P）和苯并[k]荧蒽（B[k]F）的特定分析方法。与其他22种多环芳烃（PAH）相比，在370nm激发波长和429nm发射波长下，以及在395nm激发波长和429nm发射波长下，B[a]P和B[k]F呈现出两种特定的荧光强度（高达10倍）。这些化合物在柴油尾气颗粒中的浓度是根据使用24种PAH标准混合物确定的两条校准曲线计算得出的。将这种检测方法的精密度与传统的高效液相色谱/荧光检测器（HPLC/FLD）的精密度进行了比较，我们的方法在分析标准偏差范围内给出了准确的值。本研究中开发的监测方法将使检测更加快速和简便。
