Avila L A F, Nascimento V P, Salle C T P, Moraes H L S
Center for Diagnostics and Research in Avian Pathology (CDPA), Faculty of Veterinary Medicine, Federal University of Rio Grande do Sul, Av. Bento Goncalves 8824, Porto Alegre, CEP 91540-000, Rio Grande do Sul, Brazil.
Avian Dis. 2006 Dec;50(4):608-12. doi: 10.1637/7477-112805R2.1.
The effects of probiotics and maternal vaccination with an inactivated Salmonella Enteritidis (SE) vaccine on day-old chicks challenged with SE were evaluated. A 2 X 3 factorial arrangement was used (with or without probiotics; breeders nonvaccinated, vaccinated intramuscularly, or vaccinated intraperitoneally). Three trials were conducted in isolation cabinets and SE challenge was different between trials. The number of SE organisms per chick and the time interval between housing and introduction of seeder birds (hereafter called challenge) were 1.6 X 10(8) and 1 hr (Trial I), 1.8 X 10(6) and 12 hr (Trial II), and 1.2 X 10(4) and 24 hr (Trial III). SE recovery was assessed in ceca and liver at 3, 5, and 7 days postchallenge, and the number of colony-forming units (CFU) in ceca was evaluated at 5 and 7 days postchallenge. The number of SE (log CFU) in the ceca reduced 0.56 log (from 7.59 to 7.03) and 1.45 log (7.62 to 6.17) because of the treatment with probiotics in Trials II and III, respectively. The greater reduction in Trial III indicates the importance of the early use of probiotics on the prevention of SE infection. Treatment with probiotics resulted in a smaller number of SE-positive livers after 5 days postchallenge on Trial III. Although there was no significant effect of maternal vaccination on the number of SE CFU in the ceca, a significant effect of maternal vaccination on the SE CFU was observed in the liver, but not in the ceca at 5 days after challenge.
评估了益生菌和母体接种灭活肠炎沙门氏菌（SE）疫苗对经SE攻毒的1日龄雏鸡的影响。采用2×3析因设计（有无益生菌；种鸡未接种疫苗、肌肉注射疫苗或腹腔注射疫苗）。在隔离柜中进行了3次试验，各试验的SE攻毒情况不同。每只雏鸡的SE菌数量以及雏鸡入舍与引入带菌鸡之间的时间间隔（以下简称攻毒）分别为：试验I，1.6×10⁸和1小时；试验II，1.8×10⁶和12小时；试验III，1.2×10⁴和24小时。在攻毒后3、5和7天评估盲肠和肝脏中的SE回收率，并在攻毒后5和7天评估盲肠中的菌落形成单位（CFU）数量。在试验II和III中，由于使用了益生菌，盲肠中的SE数量（log CFU）分别减少了0.56 log（从7.59降至7.03）和1.45 log（从7.62降至6.17）。试验III中更大程度的减少表明早期使用益生菌对预防SE感染的重要性。在试验III中，攻毒后5天，使用益生菌治疗使SE阳性肝脏的数量减少。尽管母体接种疫苗对盲肠中SE CFU数量没有显著影响，但在攻毒后5天，观察到母体接种疫苗对肝脏中的SE CFU有显著影响，而对盲肠没有影响。
