通过区带离心法纯化痘苗病毒并分析病毒蛋白组成。
Purification of vaccinia virus by zonal centrifugation and analysis of viral protein composition.
作者信息
Madaliński W, Bańkovski A, Korbecki M
出版信息
Acta Virol. 1977 Mar;21(2):104-8.
PMID:17277
Abstract
Vaccinia virus propagated in rotated cultures of RK13 cells was purified by sucrose density gradient zonal centrifugation. The purified virus was dissociated with sodium dodecyl sulfate end its protein composition analyzed by polyacrylamide gel electrophoresis. The protein composition of the virion envelope, isolated by the non-ionic detergent Nonidet P-40, was also determined.
摘要
在RK13细胞的旋转培养物中繁殖的痘苗病毒通过蔗糖密度梯度区带离心法进行纯化。纯化后的病毒用十二烷基硫酸钠进行解离，并通过聚丙烯酰胺凝胶电泳分析其蛋白质组成。还测定了用非离子去污剂Nonidet P - 40分离的病毒粒子包膜的蛋白质组成。
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