Brindley Paul J, Pearce Edward J
Department of Tropical Medicine, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA.
Int J Parasitol. 2007 Apr;37(5):465-73. doi: 10.1016/j.ijpara.2006.12.012. Epub 2007 Jan 4.
In contrast to the situations with model organisms and parasitic protozoa, progress with gene manipulation with schistosomes has been delayed by impediments that include our inability to maintain the life cycle in vitro, absence of immortalized cell lines, large genome sizes, unavailability of drug resistance markers and other difficulties. However, in the past few years, tangible progress has been reported towards development of tools for gene manipulation and transgenesis of schistosomes, and there is reason to believe that the field is on the verge of transformation into an era where genetic manipulation is routine. Recent reports dealing with approaches and tools to manipulate the genome and gene expression in schistosomes are reviewed here.
与模式生物和寄生原生动物的情况不同，血吸虫基因操作的进展因多种障碍而延迟，这些障碍包括我们无法在体外维持其生命周期、缺乏永生化细胞系、基因组规模大、没有耐药性标记以及其他困难。然而，在过去几年里，有报道称在血吸虫基因操作和转基因工具的开发方面取得了切实进展，而且有理由相信该领域即将迈入基因操作成为常规操作的时代。本文综述了近期有关血吸虫基因组和基因表达操作方法及工具的报道。
