• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

肾上腺皮质腺苷酸环化酶：腺苷酸环化酶及鸟苷核苷酸结合位点的溶解

Adrenal cortex adenylate cyclase: solubilization of adenylate cyclase and guanyl nucleotide binding sites.

作者信息

Glossmanm H

出版信息

Naunyn Schmiedebergs Arch Pharmacol. 1975;291(1):89-100. doi: 10.1007/BF00510823.

DOI:10.1007/BF00510823
PMID:172809
Abstract

Adenylate cyclase activity and binding sites for 5'-guanylyl-imidiphosphate [Cpp(NH)p] have been solubilized from bovine adrenal cortex membranes with Triton N-101. Solubilized adenylate cyclase is stimulated by Gpp(nh)p and fluoride ion but not by ACTH. Pretreatment of particulate membrane fractions with Gpp(NH)p, hormone and Gpp(NH)p or fluoride prior to detergent extraction leads to an activated solubilized enzyme which is nearly independent from added stimulants. Adenylate cyclase solubilized from membranes pretreated with Gpp(NH)p can be separated from guanyl nucleotide binding sites without loss of activity.

摘要

腺苷酸环化酶活性以及5'-鸟苷酰亚胺二磷酸[Cpp(NH)p]的结合位点已用曲拉通N-101从牛肾上腺皮质膜中溶解出来。溶解的腺苷酸环化酶受到Gpp(nh)p和氟离子的刺激，但不受促肾上腺皮质激素的刺激。在用去污剂提取之前，用Gpp(NH)p、激素以及Gpp(NH)p或氟对微粒体膜部分进行预处理，会产生一种活化的溶解酶，该酶几乎不依赖于添加的刺激剂。从用Gpp(NH)p预处理过的膜中溶解出来的腺苷酸环化酶可以与鸟苷酸结合位点分离，而不会丧失活性。

相似文献

1
Adrenal cortex adenylate cyclase: solubilization of adenylate cyclase and guanyl nucleotide binding sites.肾上腺皮质腺苷酸环化酶：腺苷酸环化酶及鸟苷核苷酸结合位点的溶解
Naunyn Schmiedebergs Arch Pharmacol. 1975;291(1):89-100. doi: 10.1007/BF00510823.
2
Bovine adrenal cortex adenylate cyclase: properties of the particulate enzyme and effects of guanyl nucleotides.牛肾上腺皮质腺苷酸环化酶：微粒体酶的性质及鸟苷酸的作用
Naunyn Schmiedebergs Arch Pharmacol. 1975;289(1):77-97. doi: 10.1007/BF00498031.
3
Adrenal cortex adenylate cyclase. Specific binding sites for 5'-guanylyl-imidodiphosphate in partially purified plasma membranes from bovine adrenal cortex.肾上腺皮质腺苷酸环化酶。牛肾上腺皮质部分纯化质膜中5'-鸟苷酰亚胺二磷酸的特异性结合位点。
Naunyn Schmiedebergs Arch Pharmacol. 1975;289(1):99-109. doi: 10.1007/BF00498032.
4
Adrenal cortex adenylate cyclase. In vitro acitivity of ACTH fragments and analogues.肾上腺皮质腺苷酸环化酶。促肾上腺皮质激素片段及类似物的体外活性。
Naunyn Schmiedebergs Arch Pharmacol. 1976 Aug;294(2):199-206. doi: 10.1007/BF00507854.
5
Activation of adrenal adenylate cyclase by guanine nucleotides. Promotion of nucleotide binding by calcium but not by adrenocorticotropic hormone.鸟嘌呤核苷酸对肾上腺腺苷酸环化酶的激活作用。钙可促进核苷酸结合，但促肾上腺皮质激素则无此作用。
Mol Pharmacol. 1983 Mar;23(2):369-77.
6
Solubilization and separation of the glucagon receptor and adenylate cyclase in guanine nucleotide-sensitive states.在对鸟嘌呤核苷酸敏感状态下胰高血糖素受体和腺苷酸环化酶的增溶与分离
J Biol Chem. 1977 Sep 10;252(17):5947-50.
7
5'-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells.5'-鸟苷酰亚胺二磷酸，一种真核细胞中腺苷酸环化酶系统的强效激活剂。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3087-90. doi: 10.1073/pnas.71.8.3087.
8
Adrenal cortex adenylate cyclase. Comparison between the action of GTP and 5'-guanylyl-imidodiphosphate on the particulate enzyme from bovine adrenal cortex and rat adrenals.肾上腺皮质腺苷酸环化酶。GTP与5'-鸟苷酰亚胺二磷酸对牛肾上腺皮质和大鼠肾上腺微粒体酶作用的比较。
Naunyn Schmiedebergs Arch Pharmacol. 1974;286(3):239-49. doi: 10.1007/BF00498308.
9
Multiple inhibitory and activating effects of nucleotides and magnesium on adrenal adenylate cyclase.核苷酸和镁对肾上腺腺苷酸环化酶的多种抑制和激活作用。
J Biol Chem. 1975 May 10;250(9):3459-65.
10
Hormone-receptor interactions. Stimulation and inhibition of bovine adrenal cortex cell membrane adenylate cyclase by synthetic corticotropin fragments and the effect of 5'-guanylylimidodiphosphate.激素-受体相互作用。合成促肾上腺皮质激素片段对牛肾上腺皮质细胞膜腺苷酸环化酶的刺激和抑制作用以及5'-鸟苷酰亚胺二磷酸的影响。
FEBS Lett. 1977 Jun 15;78(2):247-50. doi: 10.1016/0014-5793(77)80316-5.

引用本文的文献

1
Adrenal cortex adenylate cyclase. In vitro acitivity of ACTH fragments and analogues.肾上腺皮质腺苷酸环化酶。促肾上腺皮质激素片段及类似物的体外活性。
Naunyn Schmiedebergs Arch Pharmacol. 1976 Aug;294(2):199-206. doi: 10.1007/BF00507854.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The glucagon-sensitive adenyl cyclase system in plasma membranes of rat liver. IV. Effects of guanylnucleotides on binding of 125I-glucagon.大鼠肝脏质膜中对胰高血糖素敏感的腺苷酸环化酶系统。IV. 鸟苷酸对125I-胰高血糖素结合的影响。
J Biol Chem. 1971 Mar 25;246(6):1872-6.
3
A highly sensitive adenylate cyclase assay.一种高灵敏度的腺苷酸环化酶检测方法。
Anal Biochem. 1974 Apr;58(2):541-8. doi: 10.1016/0003-2697(74)90222-x.
4
5'-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells.5'-鸟苷酰亚胺二磷酸，一种真核细胞中腺苷酸环化酶系统的强效激活剂。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3087-90. doi: 10.1073/pnas.71.8.3087.
5
Isolation of the insulin receptor of liver and fat-cell membranes (detergent-solubilized-( 125 I)insulin-polyethylene glycol precipitation-sephadex).肝和脂肪细胞膜胰岛素受体的分离（去污剂增溶 - （125I）胰岛素 - 聚乙二醇沉淀 - 葡聚糖凝胶）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):318-22. doi: 10.1073/pnas.69.2.318.
6
Binding of 5'-guanylyl-imidodiphosphate to turkey erythrocyte membranes and effects on beta-adrenergic-activated adenylate cyclase.5'-鸟苷酰亚氨基二磷酸与火鸡红细胞膜的结合及其对β-肾上腺素能激活的腺苷酸环化酶的影响。
J Biol Chem. 1974 Dec 10;249(23):7630-6.
7
Stimulation of catecholamine-sensitive adenylate cyclase by 5'-guanylyl-imidodiphosphate.5'-鸟苷酰亚胺二磷酸对儿茶酚胺敏感的腺苷酸环化酶的刺激作用。
J Biol Chem. 1974 Oct 10;249(19):6119-24.
8
Solubilization of glucagon and epinephrine sensitive adenylate cyclase from rat liver plasma membranes.从大鼠肝细胞膜中增溶胰高血糖素和肾上腺素敏感的腺苷酸环化酶。
Biochem Biophys Res Commun. 1974 Sep 9;60(1):304-11. doi: 10.1016/0006-291x(74)90205-8.
9
Adrenal cortex adenylate cyclase. Comparison between the action of GTP and 5'-guanylyl-imidodiphosphate on the particulate enzyme from bovine adrenal cortex and rat adrenals.肾上腺皮质腺苷酸环化酶。GTP与5'-鸟苷酰亚胺二磷酸对牛肾上腺皮质和大鼠肾上腺微粒体酶作用的比较。
Naunyn Schmiedebergs Arch Pharmacol. 1974;286(3):239-49. doi: 10.1007/BF00498308.
10
The role of adenine and guanine nucleotides in the activity and response of adenylate cyclase systems to hormones: evidence for multi-site transition states.腺嘌呤和鸟嘌呤核苷酸在腺苷酸环化酶系统对激素的活性及反应中的作用：多位点过渡态的证据
Acta Endocrinol Suppl (Copenh). 1974;191:11-37. doi: 10.1530/acta.0.077s0011.