[KAI1、nm23、ETS-1、VEGF表达与鼻咽癌微血管密度的关系及临床意义]

[Relationship between the expressions of KaI1, nm23, ETS-1, VEGF and microvascular density and clinical significance in nasopharyngeal carcinoma].

作者信息

He Shu-Qian, Zhang Wei-Yuan

机构信息

Department of Pathology, Guiyang Medical College, Guiyang 550004, China.

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2006 Nov;41(11):813-7.

PMID:17283532

Abstract

OBJECTIVE

To investigate the relationship between the expressions of KAI1, nm23, ETS-1, vascular endothelial growth factor (VEGF) and microvascular density (MVD) and lymph node metastasis and prognosis in nasopharyngeal carcinoma (NPC).

METHODS

The Envision immunohistochemical method was used to detect the expressions of KAI1, nm23, ETS-1 and VEGF in 50 cases of non-keratinizing carcinoma (NKC) with cervical lymph node metastasis, 30 cases of NKC without cervical lymph node metastasis at the primary diagnoses and 30 cases of non-tumor nasopharyngeal tissues (NP). The microvascular density was counted by immunostaining with CD34.

RESULTS

(1) The expression rates of KAI1 and nm23 protein in NKC with cervical lymph node metastasis group and without cervical lymph node metastasis group and NP group increased successively , the difference being significant (P < 0.05); The expression rates of ETS-1 and VEGF protein in NKC with cervical lymph node metastasis group and without cervical lymph node metastasis group and NP group increased successively, the difference being significant (P < 0.05). (2) In 80 NKC cases, the MVD was respectively lower in KAI1 and nm23 protein positive groups than those in the negative groups (P < 0.05); the MVD was respectively higher in ETS-1 and VEGF protein positive groups than those in the negative groups (P < 0.05 ). (3) There was significant difference between the MVD, the number of NKC without cervical lymph node metastasis cases in the single expression of KAI1 or nm23 protein and in common expression of KAI1 and nm23 protein (P < 0.05), in the same as between the single expression of ETS-1 or VEGF protein and in common expressions of ETS-1 and VEGF protein (P < 0.05). (4) There was positive correlation between the expressions of KAI1 and nm23 protein (P < 0.01), as well as between the expressions of ETS-1 and VEGF protein (P < 0.01). (5) the 5-year survival rates of the patients correlated with cervical lymph node metastasis and the expressions of KAI1, nm23, ETS-1 and VEGF proteins in NKC (P < 0.05).

CONCLUSIONS

The expressions of KAI1, nm23, ETS-1 and VEGF proteins were highly related to MVD in NPC,cervical lymph node metastasis and prognosis. They might be considered to be reference indicator for evaluating the cervical lymph node metastasis and prognosis of NPC.

摘要

目的

探讨KAI1、nm23、ETS-1、血管内皮生长因子（VEGF）的表达及微血管密度（MVD）与鼻咽癌（NPC）颈部淋巴结转移及预后的关系。

方法

采用Envision免疫组化法检测50例伴有颈部淋巴结转移的非角化性癌（NKC）、30例初诊时无颈部淋巴结转移的NKC及30例鼻咽部非肿瘤组织（NP）中KAI1、nm23、ETS-1和VEGF的表达。用CD34免疫染色计数微血管密度。

结果

（1）伴有颈部淋巴结转移的NKC组、无颈部淋巴结转移的NKC组及NP组中KAI1和nm23蛋白表达率依次升高，差异有统计学意义（P<0.05）；伴有颈部淋巴结转移的NKC组、无颈部淋巴结转移的NKC组及NP组中ETS-1和VEGF蛋白表达率依次升高，差异有统计学意义（P<0.05）。（2）80例NKC病例中，KAI1和nm23蛋白阳性组的MVD分别低于阴性组（P<0.05）；ETS-1和VEGF蛋白阳性组的MVD分别高于阴性组（P<0.05）。（3）KAI1或nm23蛋白单一表达及KAI1和nm23蛋白共同表达时，MVD及无颈部淋巴结转移的NKC病例数之间差异有统计学意义（P<0.05），ETS-1或VEGF蛋白单一表达及ETS-1和VEGF蛋白共同表达时情况相同（P<0.05）。（4）KAI1和nm23蛋白表达之间呈正相关（P<0.01），ETS-1和VEGF蛋白表达之间也呈正相关（P<0.01）。（5）患者的5年生存率与颈部淋巴结转移及NKC中KAI1、nm23、ETS-1和VEGF蛋白的表达相关（P<0.05）。