[核因子-κB在烧伤血清诱导内皮细胞细胞间黏附分子-1表达中的作用]

[The role of nuclear factor-kappaB in the burn serum-induced expression of intercellular adhesion molecules-1 in endothelial cells].

作者信息

Li Zhi-qing, Huang Yue-sheng, Yang Zong-cheng, Wang Jia-han

机构信息

Department of Burns, Southern Hospital of Southern Medical University, Guangzhou 510515, P. R. China.

出版信息

Zhonghua Shao Shang Za Zhi. 2006 Oct;22(5):366-8.

PMID:17283883

Abstract

OBJECTIVE

To explore the role of nuclear factor-kappaB (NF-kappaB) in the burn serum induced expression of intercellular adhesion molecules-1 (ICAM-1) in endothelial cells.

METHODS

Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and divided into control (with normal serum stimulation), burn serum (B, with burn serum stimulation) and PDTC (with burn serum and PDTC stimulation) groups. The NF-kappaB activity in endothelial cells was determined with electrophoretic mobility shift assay (EMSA) at 0.5, 1.0, 2.0, 4.0 post-stimulation hour (PSH). The expression of ICAM-1 at 3.0, 6.0, 12. 0, 24.0 PSH was detected by flow cytometry.

RESULTS

The NF-kappaB activity in endothelial cells in burn serum group and PDTC group were markedly higher than that in control group (P <0.01), and it reached the peak at 1.0 PSH [(21.03 +/- 4.87), (7.44 +/- 0.60) x 10(4) A], respectively, then gradually decreased. But it was obviously lower in PDTC group than that in burn serum group ( P <0. 01 ). The expression of ICAM-1 was gradually increased in both burn serum group and PDTC group, reaching the peak level at 12.0 PSH [(327 +/- 37), (142 +/- 31) mean fluorescence intensity], respectively, which were significantly higher than that in control group (P <0.01). But it was evidently lower in PDTC group than that in burn serum group at 12.0 and 24.0 PSH (P <0.01).

CONCLUSION

Burn serum can initiate the synthesis and release of adhesion molecules in endothelial cells through activation of NF-kappaB, indicating that NF-kappaB plays an important role in the process of burn serum induced endothelial secretion of adhesion molecules.

摘要

目的

探讨核因子-κB（NF-κB）在烧伤血清诱导内皮细胞细胞间黏附分子-1（ICAM-1）表达中的作用。

方法

体外培养人脐静脉内皮细胞（HUVECs），分为对照组（正常血清刺激）、烧伤血清组（B组，烧伤血清刺激）和PDTC组（烧伤血清和PDTC刺激）。在刺激后0.5、1.0、2.0、4.0小时（PSH），采用电泳迁移率变动分析（EMSA）测定内皮细胞中NF-κB活性。在刺激后3.0、6.0、12.0、24.0小时，通过流式细胞术检测ICAM-1的表达。

结果

烧伤血清组和PDTC组内皮细胞中NF-κB活性显著高于对照组（P<0.01），分别在1.0 PSH时达到峰值[（21.03±4.87），（7.44±0.60）×10（4）A]，然后逐渐下降。但PDTC组明显低于烧伤血清组（P<0.01）。烧伤血清组和PDTC组ICAM-1表达均逐渐增加，分别在12.0 PSH时达到峰值水平[（327±37），（142±31）平均荧光强度]，显著高于对照组（P<0.01）。但在12.0和24.0 PSH时，PDTC组明显低于烧伤血清组（P<0.01）。