Sun Bing-Wei, Chen Xi, Chen Zhao-Yong, Kazuhiro Katada, Gediminas Cepinskas
Department of Burns and Plastic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, PR China.
Zhonghua Shao Shang Za Zhi. 2007 Jun;23(3):179-83.
To investigate the inhibitory effects of extrinsic carbon monoxide-releasing molecules II on inflammatory responses in liver of mice with severe burns and its potential mechanisms.
Forty-five male C57BL/6 mice were randomly divided into sham (simulation of burn with 37 degrees C warm water), sham + CORM-2 (with 8 mg/kg CORM-2 after the same manipulation as sham group), burn (with 15% TBSA full-thickness burns), burn + CORM-2 (with 8 mg/kg CORM-2 after the same manipulation as burn group), burn + DMSO (with DMSO after the same treatment as burn group) groups,with 9 mice in each group. The serum level of ALT and AST were determined at 24 post-burn hours (PBH), and the level of myeloperoxidase (MPO), nuclear factor (NF) kappaB, intercellular adhesion molecular (ICAM-1), vascular cell adhesion molecular (VCAM-1), as well as adhesion of polymorphonuclear leucocytes to sinusoidal endothelial cells (HSECs) after serum stimulation were detected and assessed at the same time-points.
The level of ALT and AST (398 +/- 34,122 +/- 22 ), the activity of MPO and NF-kappaB, the protein level of ICAM-1 and VCAM-1 in burn group were obviously increased when compared with those in sham group and burn + CORM-2 group (P < 0.05 or P < 0.01). Additionally, the adhesion of PMN on HSEC after stimulation of serum in burn group was enhanced, while it was markedly inhibited after stimulation of serum in burn + CORM-2 group (P < 0.05).
Extrinsic CORM-2 exhibits the ability to inhibit NF-kappaB activity, reduces the hepatic expression of ICAM-1 and VCAM-1, thereby alleviating sequestration of leukocytes after severe burns, so that hepatic inflammatory response is ameliorated, and liver function is improved.
探讨外源性一氧化碳释放分子II对严重烧伤小鼠肝脏炎症反应的抑制作用及其潜在机制。
将45只雄性C57BL/6小鼠随机分为假伤组（用37℃温水模拟烧伤）、假伤 + CORM - 2组（在与假伤组相同操作后给予8mg/kg CORM - 2）、烧伤组（15%体表面积全层烧伤）、烧伤 + CORM - 2组（在与烧伤组相同操作后给予8mg/kg CORM - 2）、烧伤 + DMSO组（在与烧伤组相同处理后给予DMSO），每组9只。于烧伤后24小时测定血清谷丙转氨酶（ALT）和谷草转氨酶（AST）水平，同时检测并评估髓过氧化物酶（MPO）、核因子（NF）κB、细胞间黏附分子（ICAM - 1）、血管细胞黏附分子（VCAM - 1）水平以及血清刺激后多形核白细胞与肝窦内皮细胞（HSECs）的黏附情况。
与假伤组和烧伤 + CORM - 2组相比，烧伤组的ALT和AST水平（398±34,122±22）、MPO和NF - κB活性、ICAM - 1和VCAM - 1蛋白水平明显升高（P < 0.05或P < 0.01）。此外，烧伤组血清刺激后PMN与HSEC的黏附增强，而烧伤 + CORM - 2组血清刺激后该黏附明显受到抑制（P < 0.05）。
外源性CORM - 2具有抑制NF - κB活性的能力，降低肝脏ICAM - 1和VCAM - 1的表达，从而减轻严重烧伤后白细胞的滞留，改善肝脏炎症反应，提高肝功能。
