Calmon Marilia F, Colombo Jucimara, Carvalho Fabrício, Souza Fátima P, Filho José F G, Fukuyama Erica E, Camargo Anamaria A, Caballero Otávia L S, Tajara Eloíza H, Cordeiro José A, Rahal Paula
Department of Biology, UNESP-IBILCE, Rua Cristovão Colombo, 2265, Jardim Nazareth, CEP: 15054-000, São José do Rio Preto, SP, Brazil.
Cancer Genet Cytogenet. 2007 Feb;173(1):31-7. doi: 10.1016/j.cancergencyto.2006.09.008.
Hypermethylation in the promoter region has been associated with a loss of gene function that may give a selective advantage to neoplastic cells. In this study, the methylation pattern of genes CDKN2A (alias p14, p14(ARF), p16, p16(INK4a)), DAPK1, CDH1, and ADAM23 was analyzed in 43 samples of head and neck tumors using methylation-specific polymerase chain reaction. In the oropharynx, there was a statistically significant association between hypermethylation of the DAPK1 gene and the occurrence of lymph node metastases, and in the larynx there was statistically significant evidence of an association between hypermethylation of the ADAM23 gene and advanced stages of the tumors. Thus, a correlation was observed between hypermethylation of the promoter region of genes DAPK1 and ADAM23 and the progression of head and neck cancer.
启动子区域的高甲基化与基因功能丧失有关，这可能赋予肿瘤细胞选择性优势。在本研究中，使用甲基化特异性聚合酶链反应分析了43例头颈部肿瘤样本中CDKN2A（别名p14、p14(ARF)、p16、p16(INK4a)）、DAPK1、CDH1和ADAM23基因的甲基化模式。在口咽中，DAPK1基因的高甲基化与淋巴结转移的发生之间存在统计学上的显著关联，而在喉中，有统计学上的显著证据表明ADAM23基因的高甲基化与肿瘤的晚期阶段有关。因此，观察到DAPK1和ADAM23基因启动子区域的高甲基化与头颈部癌的进展之间存在相关性。
