海洋无脊椎动物C型凝集素的多种糖结合特异性

Diverse sugar-binding specificities of marine invertebrate C-type lectins.

作者信息

Matsubara Hiroki, Nakamura-Tsuruta Sachiko, Hirabayashi Jun, Jimbo Mitsuru, Kamiya Hisao, Ogawa Tomohisa, Muramoto Koji

机构信息

Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.

出版信息

Biosci Biotechnol Biochem. 2007 Feb;71(2):513-9. doi: 10.1271/bbb.60543. Epub 2007 Feb 7.

DOI:10.1271/bbb.60543

PMID:17284851

Abstract

The sugar-binding specificities of C-type lectins isolated from marine invertebrates were investigated by frontal affinity chromatography (FAC) using 100 oligosaccharides. The lectins included BRA-2 and BRA-3, multiple lectins from the hemolymph of the acorn barnacle, Megabalanus rosa, and BRL from the acorn barnacle, Balanus rostatus. The diverse sugar-binding specificities of the C-type lectins were determined by FAC analysis. BRA-2 recognized alpha2-6 sialylation but not alpha2-3 sialylation on glycans. On the other hand, BRA-3 showed high affinity for oligosaccharides with alpha-linked non-reducing terminal galactose, but not for sialylated forms, and BRL showed enhanced recognition activity towards Lewis(x) and Lewis(a) epitopes.

摘要

利用100种寡糖，通过前沿亲和色谱法（FAC）研究了从海洋无脊椎动物中分离出的C型凝集素的糖结合特异性。这些凝集素包括BRA - 2和BRA - 3，它们是从玫瑰巨藤壶血淋巴中分离出的多种凝集素，以及从罗氏藤壶中分离出的BRL。通过FAC分析确定了C型凝集素多样的糖结合特异性。BRA - 2识别聚糖上的α2 - 6唾液酸化，但不识别α2 - 3唾液酸化。另一方面，BRA - 3对具有α连接的非还原末端半乳糖的寡糖具有高亲和力，但对唾液酸化形式没有亲和力，并且BRL对Lewis(x)和Lewis(a)表位表现出增强的识别活性。