[γ-干扰素对伤口愈合和瘢痕形成作用中蛋白激酶C的活性变化]
[Activity changes of protein kinase C in effect of interferon-gamma on wound healing and cicatrisation].
作者信息
Zhang Xuan-fen, Guo Shu-zhong, Zhang Lin-xi, Liu Kai
机构信息
Department of Plastic Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2006 Nov;22(6):442-4.
OBJECTIVE
To study the roles of protein kinase C (PKC) in effect of interferon-gamma (IFN-gamma) on wound healing and cicatrization.
METHODS
IFN-gamma was applied on the wound and into the scar tissues of rabbit ear before or after wound healing. PKC activities in the tissues from 0, 3, 6 d, 11-16 d post-wounding and from 14, 30 and 45d post-epithelization were measured by phosphorus (32p) incorporation. The time of wound epithelization and scar changes were also observed.
RESULTS
The PKC activity in granulation tissue, wound margin tissue and scar tissue elevated obviously in comparing with that of normal skin (P < 0.01). IFN-gamma did not change PKC activity (P > 0.05). But it delayed the wound healing (P < 0.01) and inhibited scar hyperplasia (P <0.05).
CONCLUSIONS
PKC might not mediate the signal of IFN-gamma inhibiting the wound healing and scar hyperplasia. But PKC might be related to the wound healing and scar hyperplasia.
目的
研究蛋白激酶C（PKC）在干扰素-γ（IFN-γ）对伤口愈合及瘢痕形成影响中的作用。
方法
在伤口愈合前或愈合后，将IFN-γ应用于兔耳伤口及瘢痕组织。采用磷（32P）掺入法测定伤后0、3、6天，11 - 16天以及上皮化后14、30和45天组织中的PKC活性。同时观察伤口上皮化时间及瘢痕变化。
结果
与正常皮肤相比，肉芽组织、伤口边缘组织及瘢痕组织中的PKC活性明显升高（P < 0.01）。IFN-γ未改变PKC活性（P > 0.05）。但它延迟了伤口愈合（P < 0.01）并抑制了瘢痕增生（P < 0.05）。
结论
PKC可能不介导IFN-γ抑制伤口愈合及瘢痕增生的信号。但PKC可能与伤口愈合及瘢痕增生有关。
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