Biswas R, Chattopadhyay U
Department of Tumor Immunobiology, Chittaranjan National Cancer Institute, Calcutta, India.
Int J Cancer. 1992 Jan 2;50(1):93-8. doi: 10.1002/ijc.2910500119.
Interaction of prolactin and glucocorticoid on thymocytes and splenocytes of normal and tumor-bearing mice with reference to their mitogen-responsive blastogenesis has been studied. Prolactin alone had little or no mitogenic effect on thymocytes and splenocytes of normal mice but it was co-stimulatory, with ConA, in inducing blastogenesis in normal splenocytes. Thymocytes and splenocytes of tumor-bearing mice responded differently to prolactin. Hormone alone inhibited growth of thymocytes but at certain concentrations stimulated splenocytes. When cultured with prolactin and ConA, the thymocytes of tumor hosts responded with increased proliferation compared to that induced by ConA alone. Glucocorticoid suppressed ConA-induced lymphocyte proliferation in both normal and tumor-bearing mice. Prolactin reversed the inhibitory effect of glucocorticoid in normal mice but failed to abrogate inhibition in tumor hosts. Altered responsiveness of lymphocytes of tumor hosts to prolactin was not a function of circulating prolactin, as the serum prolactin level was similar in normal and tumor-bearing mice. Prolactin, however, could not reverse estradiol-induced suppression of lymphocyte proliferation. The lactogenic hormone, but not somatogenic hormones, altered the glucocorticoid inhibition of lymphocyte growth.
研究了催乳素和糖皮质激素对正常小鼠及荷瘤小鼠胸腺细胞和脾细胞的相互作用，及其与有丝分裂原反应性细胞增殖的关系。单独的催乳素对正常小鼠的胸腺细胞和脾细胞几乎没有或没有促有丝分裂作用，但在诱导正常脾细胞的细胞增殖方面，它与刀豆蛋白A（ConA）具有协同刺激作用。荷瘤小鼠的胸腺细胞和脾细胞对催乳素的反应不同。单独的激素抑制胸腺细胞生长，但在一定浓度下刺激脾细胞。当与催乳素和ConA一起培养时，与单独使用ConA诱导的增殖相比，荷瘤宿主的胸腺细胞增殖增加。糖皮质激素抑制正常小鼠和荷瘤小鼠中ConA诱导的淋巴细胞增殖。催乳素逆转了糖皮质激素对正常小鼠的抑制作用，但未能消除对荷瘤宿主的抑制作用。荷瘤宿主淋巴细胞对催乳素反应性的改变不是循环催乳素的作用，因为正常小鼠和荷瘤小鼠的血清催乳素水平相似。然而，催乳素不能逆转雌二醇诱导的淋巴细胞增殖抑制。催乳激素而非生长激素改变了糖皮质激素对淋巴细胞生长的抑制作用。
