Brutkiewicz Susan, Mendonca Marc, Stantz Keith, Comerford Kathleen, Bigsby Robert, Hutchins Gary, Goebl Mark, Harrington Maureen
Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Luminescence. 2007 May-Jun;22(3):221-8. doi: 10.1002/bio.953.
In vivo bioluminescence imaging is becoming a very important tool for the study of a variety of cellular and molecular events or disease processes in living systems. In vivo bioluminescence imaging is based on the detection of light emitted from within an animal. The light is generated as a product of the luciferase-luciferin reaction taking place in a cell. In this study, we implanted mice with tumour cells expressing either a high or a low level of luciferase. In vivo bioluminescence imaging was used to follow tumour progression. Repeated luciferin injection and imaging of high and low luciferase-expressing tumours was performed. While low luciferase-expressing tumours grew similarly to vector controls, growth of the high luciferase-expressing tumours was severely inhibited. The observation that a high level of luciferase expression will inhibit tumour cell growth when an animal is subjected to serial in vivo bioluminescence imaging is potentially an important factor in designing these types of studies.
体内生物发光成像正成为研究活体系统中各种细胞和分子事件或疾病过程的一项非常重要的工具。体内生物发光成像基于对动物体内发出的光的检测。这种光是细胞内发生的荧光素酶-荧光素反应的产物。在本研究中，我们给小鼠植入了表达高水平或低水平荧光素酶的肿瘤细胞。利用体内生物发光成像来跟踪肿瘤进展。对表达高荧光素酶和低荧光素酶的肿瘤进行了重复的荧光素注射和成像。虽然表达低荧光素酶的肿瘤生长情况与载体对照相似，但表达高荧光素酶的肿瘤生长受到严重抑制。当动物接受系列体内生物发光成像时，高水平的荧光素酶表达会抑制肿瘤细胞生长，这一观察结果可能是设计这类研究的一个重要因素。
