Majewski S, Hunzelmann N, Schirren C G, Mauch C, Aumailley M, Krieg T
Department of Dermatology, University of Cologne, Federal Republic of Germany.
J Invest Dermatol. 1992 Jan;98(1):86-91. doi: 10.1111/1523-1747.ep12495664.
A characteristic feature of systemic scleroderma is fibrosis of the skin and eventually of internal organs resulting from an overproduction of collagen and other connective tissue components by the resident fibroblasts. The balance between the cells and the amount of the surrounding extracellular matrix is then altered. Because cellular metabolism depends to a large extent on cellular contacts and communications with connective tissue molecules, we have therefore investigated the interactions with extracellular matrix components of fibroblasts obtained from skin of patients affected with scleroderma. In comparison to fibroblasts from healthy skin, all fibroblasts from scleroderma patients had an increased adhesion capacity to collagens I, IV, VI, fibronectin, and laminin. In addition, whereas adhesion of control fibroblasts was stimulated by a pre-treatment with transforming growth factor-beta, adhesion patterns of scleroderma fibroblasts remained unchanged. However, pre-incubation of the cells with interferon-gamma decreased the adhesion of both scleroderma and control fibroblasts.
系统性硬皮病的一个特征是皮肤纤维化，最终导致内部器官纤维化，这是由驻留成纤维细胞过度产生胶原蛋白和其他结缔组织成分所致。细胞与周围细胞外基质的量之间的平衡随即发生改变。由于细胞代谢在很大程度上依赖于细胞与结缔组织分子的接触和通讯，因此我们研究了硬皮病患者皮肤来源的成纤维细胞与细胞外基质成分之间的相互作用。与健康皮肤来源的成纤维细胞相比，硬皮病患者的所有成纤维细胞对I型、IV型、VI型胶原蛋白、纤连蛋白和层粘连蛋白的黏附能力均增强。此外，对照成纤维细胞的黏附受到转化生长因子-β预处理的刺激，而硬皮病成纤维细胞的黏附模式则保持不变。然而，用γ干扰素对细胞进行预孵育会降低硬皮病成纤维细胞和对照成纤维细胞的黏附。
