[醛固酮对培养的大鼠系膜细胞中纤连蛋白合成及转化生长因子-β1 mRNA表达的影响]

[Effects of aldosterone on synthesis of fibronectin and expression of transforming growth factor-beta1 mRNA in cultured rat mesangial cells].

作者信息

Li Xiao-dong, Chen Xiao-wen, Tang De-shen, Liang Dong, Liu Hua-feng

机构信息

Institute of Nephrology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Feb;23(2):140-2.

PMID:17286908

Abstract

AIM

To evaluate the effect of aldosterone (ALD) and spironolactone (SPI, one of the aldosterone receptor antagonists) on synthesis and secretion of fibronectin (FN) and expression of transforming growth factor-beta1 (TGF-beta1) mRNA in cultured rat mesangial cells.

METHODS

(1) Mesangial cells were treated with medium containing different concentrations of ALD (10(-11), 10(-9), 10(-7) mol/L) and/or 10(-7) mol/L SPI for 48 h, while control cells were treated with vehicle only. The levels of FN in the supernatants were measured by ELISA method. The expressions of FN mRNA and TGF-beta1 mRNA were detected by semi-quantitative RT-PCR. (2) Mesangial cells were treated with 10(-9) mol/L ALD for 24, 48, 72 h. The levels of FN in the supernatants were measured by ELISA method. The expressions of FN mRNA and TGF-beta1 mRNA were detected by semi-quantitative RT-PCR.

RESULTS

ELISA method showed that the level of FN in the supernatant of cultured rat mesangial cells stimulated with ALD increased significantly in a dose- and time-dependent manner. Also the expressions of FN mRNA and TGF-beta1 mRNA were increased significantly by ALD in a dose- and time-dependent manner by semi-quantitative RT-PCR. SPI inhibited the stimulating effect of ALD on synthesis of FN and expressions of FN mRNA and TGF-beta1 mRNA in cultured rat mesangial cells.

CONCLUSION

ALD up-regulates the protein synthesis and mRNA expression of FN, and up-regulates the mRNA expression of TGF-beta1 in cultured rat mesangial cells. SPI inhibits the effect of ALD, and thus implication in the treatment of Glomerulosclerosis.

摘要

目的

评估醛固酮（ALD）及醛固酮受体拮抗剂之一螺内酯（SPI）对培养的大鼠系膜细胞中纤连蛋白（FN）合成与分泌及转化生长因子-β1（TGF-β1）mRNA表达的影响。

方法

（1）用含不同浓度ALD（10⁻¹¹、10⁻⁹、10⁻⁷mol/L）和/或10⁻⁷mol/L SPI的培养基处理系膜细胞48小时，而对照细胞仅用溶剂处理。用ELISA法检测上清液中FN的水平。用半定量RT-PCR检测FN mRNA和TGF-β1 mRNA的表达。（2）用10⁻⁹mol/L ALD处理系膜细胞24、48、72小时。用ELISA法检测上清液中FN的水平。用半定量RT-PCR检测FN mRNA和TGF-β1 mRNA的表达。

结果

ELISA法显示，用ALD刺激的培养大鼠系膜细胞上清液中FN水平呈剂量和时间依赖性显著增加。半定量RT-PCR也显示，ALD使FN mRNA和TGF-β1 mRNA的表达呈剂量和时间依赖性显著增加。SPI抑制ALD对培养的大鼠系膜细胞中FN合成及FN mRNA和TGF-β1 mRNA表达的刺激作用。