Department of Pediatric Nephrology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210003, P.R. China.
Division of Nephrology, Huashan Hospital and Institute of Nephrology, Fudan University, Shanghai 200040, P.R. China.
Mol Med Rep. 2017 Nov;16(5):6004-6012. doi: 10.3892/mmr.2017.7313. Epub 2017 Aug 22.
Clinical and experimental reports indicate that aldosterone (ALD) contributes to the progression of renal failure independent of its hemodynamic effects. However, the mechanisms remain to be completely elucidated. The aim of the present study was to investigate the alterations of long non‑coding RNA (lncRNA) in mesangial cells (MCs) treated with ALD. The present study used MCs treated with 10‑6 M ALD as experimental cells. Microarray techniques performed by Agilent Technologies were used to identify the profiles of differentially expressed lncRNAs between the ALD group and the control group. Pathway and gene ontology analysis were applied to determine the roles of the differentially expressed lncRNAs. Reverse transcription quantitative polymerase chain reaction (RT‑qPCR) was used to quantify the differentially expressed lncRNAs. A total of 8,459 lncRNA and 13,214 mRNAs with differential expression between MCs treated with and without ALD were identified. The expression of lncRNAs was confirmed by RT‑qPCR and the results were consistent with the lncRNA array. The biological functions of lncRNAs are associated with responding to external stimuli, positive regulation of biological and apoptotic processes, cell division, mitosis and nuclear division. The pathways include cell cycle and peroxisome proliferator‑activated receptor signaling pathways. The present study revealed distinct sets of lncRNA expressed in MCs treated with ALD, suggesting that this class of transcripts may be involved in the pathogenesis of chronic kidney diseases.
临床和实验报告表明，醛固酮（ALD）独立于其血液动力学效应促进肾衰竭的进展。然而，其机制仍有待完全阐明。本研究旨在探讨ALD 处理的肾小球系膜细胞（MCs）中长链非编码 RNA（lncRNA）的变化。本研究使用 10-6 M ALD 处理的 MCs 作为实验细胞。使用 Agilent Technologies 进行的微阵列技术用于鉴定 ALD 组和对照组之间差异表达的 lncRNA 图谱。通路和基因本体分析用于确定差异表达的 lncRNA 的作用。逆转录定量聚合酶链反应（RT-qPCR）用于定量差异表达的 lncRNA。在 ALD 处理和未处理的 MCs 之间鉴定出 8459 个 lncRNA 和 13214 个差异表达的 mRNA。通过 RT-qPCR 证实了 lncRNA 的表达，结果与 lncRNA 阵列一致。lncRNA 的生物学功能与对外界刺激的反应、生物和凋亡过程的正调节、细胞分裂、有丝分裂和核分裂有关。通路包括细胞周期和过氧化物酶体增殖物激活受体信号通路。本研究揭示了 ALD 处理的 MCs 中表达的不同 lncRNA 集，表明这类转录物可能参与慢性肾脏病的发病机制。
