Ueda Katsura, Ichimori Yasuo, Okada Hiroyuki, Honma Shiho, Wakisaka Satoshi
Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Japan.
Arch Histol Cytol. 2006 Dec;69(4):289-96. doi: 10.1679/aohc.69.289.
Double immunohistochemistry of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins [synaptosomal-associated protein of 25 kDa (SNAP-25), syntaxin and vesicle-associated protein-2 (VAMP-2)], and specific cell markers of taste buds cells [alpha-gustducin and phospholipase Cbeta2 (PLCbeta2) for type II cells; neural cell adhesion molecule (NCAM) for type III cells] was applied to gustatory epithelia of the rat circumvallate papillae. All three SNARE proteins were present in some elongated taste buds cells as well as intra-, peri- and subgemmal nerve fibers. Double immunohisotochemistry revealed that nearly all alpha-gustducin and PLCbeta2 immunoreactive cells expressed SNAP-25, syntaxin, and VAMP-2. A majority of NCAM immunoreactive cells showed immunoreactivity for these SNARE proteins. These results indicate that these synapse-associated proteins (SNAP-25, syntaxin and VAMP-2) are present in both type II cells and type III cells. Moreover, more than 50% of intragemmal cells containing SNARE proteins showed immunoreactivities for alpha-gustducin, PLCbeta2, and NCAM, suggesting the possible presence of transitional cells having histochemical properties of both type II and type III cells.
对可溶性N - 乙基马来酰亚胺敏感融合蛋白附着蛋白受体（SNARE）蛋白[25 kDa的突触体相关蛋白（SNAP - 25）、 syntaxin和囊泡相关蛋白 - 2（VAMP - 2）]以及味蕾细胞的特异性细胞标志物[II型细胞的α - 味导素和磷脂酶Cβ2（PLCβ2）；III型细胞的神经细胞黏附分子（NCAM）]进行双重免疫组化，应用于大鼠轮廓乳头的味觉上皮。所有这三种SNARE蛋白都存在于一些细长的味蕾细胞以及味蕾内、周围和味蕾下神经纤维中。双重免疫组化显示，几乎所有α - 味导素和PLCβ2免疫反应性细胞都表达SNAP - 25、 syntaxin和VAMP - 2。大多数NCAM免疫反应性细胞对这些SNARE蛋白也有免疫反应性。这些结果表明，这些突触相关蛋白（SNAP - 25、 syntaxin和VAMP - 2）存在于II型细胞和III型细胞中。此外，超过50%含有SNARE蛋白的味蕾内细胞对α - 味导素、PLCβ2和NCAM有免疫反应性，提示可能存在具有II型和III型细胞组织化学特性的过渡细胞。
