通过光纤探头和血氧水平依赖性功能磁共振成像评估糖尿病早期肾髓质血流动力学变化。
Early changes with diabetes in renal medullary hemodynamics as evaluated by fiberoptic probes and BOLD magnetic resonance imaging.
作者信息
dos Santos Elisabete Alcantara, Li Lu-Ping, Ji Lin, Prasad Pottumarthi Vara
机构信息
Center for Advanced Imaging, Department of Radiology, Evanston Northwestern Healthcare, Evanston, IL 60201, USA.
出版信息
Invest Radiol. 2007 Mar;42(3):157-62. doi: 10.1097/01.rli.0000252492.96709.36.
Abstract
OBJECTIVE
We sought to evaluate the influence of streptozotocin (STZ)-induced diabetes on renal outer medullary pO2 and blood flow by invasive microprobes and to demonstrate feasibility that blood oxygenation level-dependent (BOLD) magnetic resonance imaging (MRI) can monitor these changes.
MATERIALS AND METHODS
A total of 60 Wistar-Furth rats were used. Diabetes was induced by STZ in 48. Animals were divided into OxyLite group (n=30) and BOLD MRI groups (n=30) each with a 5 subgroups of 6 animals: control and 2, 5, 14, and 28 days after induction of diabetes. Outer renal medullary oxygen tension and blood flow were measured by the combined OxyLite/OxyFlo probes.
RESULTS
Both OxyLite and BOLD MRI showed a significant increase in the renal hypoxia levels after STZ at all time points. However, no changes were observed in the outer renal medullary oxygen tension and blood flow between diabetic and control groups.
CONCLUSIONS
These preliminary results suggest that hypoxic changes can be detected as early as 2 days in rat kidneys with diabetes by BOLD MRI and that these early changes are not dependent on blood flow.
摘要
目的
我们试图通过侵入性微探针评估链脲佐菌素（STZ）诱导的糖尿病对肾外髓质氧分压（pO2）和血流的影响，并证明血氧水平依赖（BOLD）磁共振成像（MRI）监测这些变化的可行性。
材料与方法
总共使用60只Wistar-Furth大鼠。48只大鼠通过STZ诱导糖尿病。动物分为氧分压（OxyLite）组（n = 30）和BOLD MRI组（n = 30），每组各有5个亚组，每组6只动物：对照组以及糖尿病诱导后2天、5天、14天和28天组。使用OxyLite/OxyFlo组合探针测量肾外髓质氧张力和血流。
结果
OxyLite和BOLD MRI均显示，在所有时间点，STZ诱导后肾脏缺氧水平显著升高。然而，糖尿病组和对照组之间肾外髓质氧张力和血流未观察到变化。
结论
这些初步结果表明，BOLD MRI可在糖尿病大鼠肾脏中最早于2天检测到缺氧变化，且这些早期变化不依赖于血流。
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