Bray D P, Bown K J, Stockley P, Hurst J L, Bennett M, Birtles R J
Mammalian Behaviour and Evolution Group, Faculty of Veterinary Science, University of Liverpool, Leahurst, Chester Road, Neston, Cheshire CH64 7TE, UK.
Parasitology. 2007 Jun;134(Pt 6):819-26. doi: 10.1017/S0031182007002302. Epub 2007 Feb 9.
The presence of haemoparasites belonging to the taxa Anaplasma, Bartonella and Trypanosoma was determined among 76 common shrews (Sorex araneus) from Northwest England. Anaplasma phagocytophilum DNA was recovered from the blood of 1 shrew (1.3%), with the amplified 16S rRNA sequence identical to one previously reported from a bank vole (Clethrionomys glareolus). Trypanosoma spp. DNA was detected in 9 shrews (11.8%), the amplified 18S rDNA fragments being indistinguishable from one another, and distinct from previously published data. This represents the first report of trypanosome infection in S. araneus and suggests they are susceptible to an uncharacterized Trypanosoma species. Blood from 11 shrews (14.5%) yielded Bartonella spp., with characterization of isolates using comparative sequence analysis of partial gltA and 16S-23S rRNA intergenic spacer regions revealing 2 different genotypes. Phylogenetic inference from alignment of partial gltA sequences found that both UK S. araneus types formed a well-supported cluster with Bartonella sp. isolated from S. araneus in Sweden. No significant effect of host age, sex, or year of collection was found on prevalence of Bartonella or trypanosome infections. The results of this survey demonstrate that common shrews in the UK are susceptible to haemoparasitic infections, at prevalences similar to those reported from sympatric rodents.
在来自英格兰西北部的76只普通鼩鼱（Sorex araneus）中，检测了属于无形体属、巴尔通体属和锥虫属的血液寄生虫的存在情况。从1只鼩鼱（1.3%）的血液中检测到嗜吞噬细胞无形体DNA，扩增的16S rRNA序列与先前从长尾田鼠（Clethrionomys glareolus）报道的序列相同。在9只鼩鼱（11.8%）中检测到锥虫属DNA，扩增的18S rDNA片段彼此无法区分，且与先前发表的数据不同。这是首次在普通鼩鼱中报道锥虫感染，表明它们易感染一种未鉴定的锥虫物种。11只鼩鼱（14.5%）的血液检测出巴尔通体属，通过对部分gltA和16S - 23S rRNA基因间隔区进行比较序列分析对分离株进行鉴定，发现了2种不同的基因型。根据部分gltA序列比对进行的系统发育推断发现，英国普通鼩鼱的两种类型与从瑞典普通鼩鼱分离出的巴尔通体形成了一个有充分支持的聚类。未发现宿主年龄、性别或采集年份对巴尔通体或锥虫感染率有显著影响。本次调查结果表明，英国的普通鼩鼱易感染血液寄生虫，感染率与同域啮齿动物报道的相似。
