一种糖皮质激素受体的部分纯化
Partial purification of a glucocorticoid receptor.
作者信息
Failla D, Tomkins G M, Santi D V
出版信息
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3849-52. doi: 10.1073/pnas.72.10.3849.
Abstract
A simple method for purification of the glucocorticoid receptor from hepatoma tissue culture cells has been developed. The procedure, which requires only about 24 hr, involves biospecific adsorption of the receptor to deoxycorticosterone derivatized agarose, elution with a glucocorticoid, and gel filtration. The receptor-steroid complex is obtained in 35-40% yield and is about 2000-fold purified. It possesses properties similar to those reported in crude extracts, including sedimentation coefficient in glycerol gradients and activation-dependent binding to nuclei.
摘要
已开发出一种从肝癌组织培养细胞中纯化糖皮质激素受体的简单方法。该方法仅需约24小时，包括将受体生物特异性吸附到脱氧皮质酮衍生的琼脂糖上，用糖皮质激素洗脱，以及凝胶过滤。受体 - 类固醇复合物的产率为35 - 40%，纯化倍数约为2000倍。它具有与粗提物中报道的性质相似的特性，包括在甘油梯度中的沉降系数以及与细胞核的激活依赖性结合。
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