[Genotype of TEM- and SHV-type beta-lactamase producing Klebsiella pneumoniae in Guangzhou area].

OBJECTIVE

To determine the prevalence and genotypes of TEM- and SHV-type beta-lactamase-producing Klebsiella pneumoniae in Guangzhou area.

METHODS

Genotype determination of TEM- and SHV-type beta-lactamases were performed by PCR amplification followed by denaturing high-performance liquid chromatography (DHPLC) and DNA sequencing among 57 strain of klebsiella pneumonia obtained from the hospitals in Guangzhou.

RESULTS

The genes bla(TEM) and bla(SHV) were amplified from all strains. TEM-116 type ESBLs were found in 56.14% (32/57) of the isolates. SHV-12 (n = 19), -11 (n = 11), -2a (n = 6), -28 (n = 4), -26 (n = 1), -33 (n = 1) and five novel SHV-type beta-lactamases were identified among the SHV-type beta-lactamases. These novel enzymes were designated from SHV-64, -65, -66, -67 and SHV-69, which derived from SHV-1 beta-lactamase or SHV-12 ESBL. A total of 94.74% of the isolates (54/57) produced both TEM- and SHV-type beta-lactamases simultaneously, in which 13.0% were TEM-116 and SHV-12 (n = 12).

CONCLUSION

TEM-116 and SHV-12 are the predominant TEM- and SHV-type genotypes of Klebsiella pneumoniae producing beta-lactamases in Guangzhou. This area is confronted with the prevalence of the phenotypes of Klebsiella pneumoniae that simultaneously produce TEM- and SHV-type beta-lactamases.