[上调前列腺癌细胞系LNCaP中maspin基因的表达]

[Up-regulates the expression of maspin gene in prostate cancer cell line LNCaP].

作者信息

Shi Ping, Chen Wei-Wen, Hu Xiao-Yan, Yu Chun-Xiao, Zhang Peng-Ju, Jiang An-Li, Zhang Jian-Ye

机构信息

Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China.

出版信息

Yao Xue Xue Bao. 2006 Dec;41(12):1152-6.

PMID:17290611

Abstract

AIM

To study the effect of curcumin on the apoptosis of prostate cancer cell line LNCaP and regulation of expression of maspin gene.

METHODS

MTT and DNA electrophoresis were used to examine the cell growth and apoptosis of prostate cancer cell line LNCaP after treated with different doses of curcumin. The expression of maspin gene at transcription level and translation level was also detected by RT-PCR and Western blotting. pGL3-maspin luciferase expression vector, containing 847 bp (- 764 -/+ 83) DNA of maspin gene 5' promoter region, was transient transfected into LNCaP cell. Through detecting the activity of luciferase, the effect of curcumin on the promoter of maspin was studied.

RESULTS

Curcumin inhibited cell growth, induced the apoptosis and enhanced the expression of maspin gene in LNCaP cells.

CONCLUSION

Curcumin up-regulated expression of maspin gene in LNCaP cells through enhancing the transcription activity of promoter of maspin gene.

摘要

目的

研究姜黄素对前列腺癌细胞系LNCaP凋亡及maspin基因表达调控的影响。

方法

采用MTT法和DNA电泳检测不同剂量姜黄素处理后前列腺癌细胞系LNCaP的细胞生长及凋亡情况。同时运用RT-PCR和蛋白质印迹法检测maspin基因在转录水平和翻译水平的表达。将含有maspin基因5'启动子区847 bp（-764-/+83）DNA的pGL3-maspin荧光素酶表达载体瞬时转染至LNCaP细胞。通过检测荧光素酶活性，研究姜黄素对maspin启动子的作用。