Gente Stéphanie, Larpin Sandra, Cholet Orianne, Guéguen Micheline, Vernoux Jean-Paul, Desmasures Nathalie
Laboratoire de Microbiologie Alimentaire, Université de Caen Basse-Normandie, Esplanade de la paix, 14032 Caen cedex, France.
J Dairy Res. 2007 May;74(2):137-45. doi: 10.1017/S0022029906002226. Epub 2007 Feb 12.
PCR primers were developed for the specific detection of Clavispora lusitaniae, Debaryomyces hansenii var hansenii, Geotrichum candidum, Kluyveromyces lactis and K. marxianus and Yarrowia lipolytica, yeast species commonly found on the surface of smear cheese. Forty eight representative strains frequently found in smear cheeses or taxonomically related to the target yeasts were used as templates, to validate the designed primers. The specific and selective detection of these yeasts was effective in situ, in Livarot smear, without yeast isolation and culture and was comparable with data obtained with a conventional method. The primers described here have thus potential for PCR studies applied to cheese. It should also be possible to use some of these primers with other substrates.
开发了聚合酶链反应（PCR）引物，用于特异性检测葡萄牙棒孢酵母、汉逊德巴利酵母汉逊变种、白地霉、乳酸克鲁维酵母、马克斯克鲁维酵母和解脂耶氏酵母，这些都是涂抹干酪表面常见的酵母菌种。48株在涂抹干酪中经常发现或与目标酵母在分类学上相关的代表性菌株用作模板，以验证设计的引物。这些酵母的特异性和选择性检测在原位、在利瓦罗涂抹干酪中有效，无需酵母分离和培养，并且与传统方法获得的数据相当。因此，这里描述的引物具有应用于奶酪的PCR研究潜力。使用其中一些引物用于其他底物也应该是可行的。
