在沙鼠黑质网状部中无法检测到神经元特异性核抗原NeuN。
Neuron-specific nuclear antigen NeuN is not detectable in gerbil subtantia nigra pars reticulata.
作者信息
Kumar Sanjay S, Buckmaster Paul S
机构信息
Department of Comparative Medicine, Stanford University, Stanford, CA 94305-5342, USA.
出版信息
Brain Res. 2007 Apr 20;1142:54-60. doi: 10.1016/j.brainres.2007.01.027. Epub 2007 Jan 13.
Abstract
NeuN (Neuronal Nuclei), the neuron-specific marker of nuclear protein is used extensively in histological procedures to identify major cell-types in adult vertebrate nervous systems of a variety of species including rodents and humans. Some notable exceptions (i.e., NeuN-negative neurons) include Purkinje cells in cerebellum, mitral cells in olfactory bulb, and photoreceptors in retina. Here we report that neurons in gerbil (Meriones unguiculatus) substantia nigra pars reticulata (SNr), whose "neuronal" phenotype was confirmed via electrophysiology, biocytin-labeling, histology, and in situ hybridization, are also devoid of NeuN-immunoreactivity as assayed with the widely used monoclonal antibody A60. Immunohistochemistry of rat SNr using the same antibody yielded robust staining. These data suggest lack of NeuN-immonoreactivity observed in certain cell-types and brain regions can be species-specific.
摘要
NeuN（神经元细胞核）是一种核蛋白的神经元特异性标志物，在组织学程序中被广泛用于识别包括啮齿动物和人类在内的多种物种成年脊椎动物神经系统中的主要细胞类型。一些显著的例外情况（即NeuN阴性神经元）包括小脑的浦肯野细胞、嗅球的二尖瓣细胞和视网膜的光感受器。在此我们报告，通过电生理学、生物素标记、组织学和原位杂交证实其“神经元”表型的沙鼠（长爪沙鼠）黑质网状部（SNr）中的神经元，在用广泛使用的单克隆抗体A60检测时也缺乏NeuN免疫反应性。使用相同抗体对大鼠SNr进行免疫组织化学染色产生了强烈的染色。这些数据表明，在某些细胞类型和脑区中观察到的NeuN免疫反应性缺乏可能具有物种特异性。
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本文引用的文献
1
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J Neurophysiol. 2006 Apr;95(4):2446-55. doi: 10.1152/jn.01173.2005. Epub 2006 Jan 11.
2
Loss of NeuN immunoreactivity after cerebral ischemia does not indicate neuronal cell loss: a cautionary note.
Brain Res. 2004 Jul 23;1015(1-2):169-74. doi: 10.1016/j.brainres.2004.04.032.
3
Developmental and cell type-specific expression of the neuronal marker NeuN in the murine cerebellum.
J Neurosci Res. 2003 Aug 1;73(3):400-9. doi: 10.1002/jnr.10655.
4
Sexual dimorphism and developmental regulation of substantia nigra function.
Ann Neurol. 2001 Nov;50(5):596-601. doi: 10.1002/ana.1248.
5
Low microsatellite variation in laboratory gerbrils.
J Hered. 2001 Jan-Feb;92(1):71-4. doi: 10.1093/jhered/92.1.71.
6
GLIA: listening and talking to the synapse.
Nat Rev Neurosci. 2001 Mar;2(3):185-93. doi: 10.1038/35058528.
7
Neuronal nuclear antigen (NeuN): a marker of neuronal maturation in early human fetal nervous system.
Brain Dev. 1998 Mar;20(2):88-94. doi: 10.1016/s0387-7604(97)00111-3.
8
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Neuroscience. 1997 Sep;80(2):545-57. doi: 10.1016/s0306-4522(97)00093-6.
9
Heterogeneity of astrocyte resting membrane potentials and intercellular coupling revealed by whole-cell and gramicidin-perforated patch recordings from cultured neocortical and hippocampal slice astrocytes.
J Neurosci. 1997 Sep 15;17(18):6850-63. doi: 10.1523/JNEUROSCI.17-18-06850.1997.
10
The calretinin-containing mossy cells survive excitotoxic insult in the gerbil dentate gyrus. Comparison of excitotoxicity-induced neuropathological changes in the gerbil and rat.
Eur J Neurosci. 1996 Nov;8(11):2371-8. doi: 10.1111/j.1460-9568.1996.tb01200.x.
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