Lu Qiang, Zhang Shenjia, Hu Kun, Feng Qingling, Cao Chuanbao, Cui Fuzhai
Laboratory of Advanced Materials, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Biomaterials. 2007 May;28(14):2306-13. doi: 10.1016/j.biomaterials.2007.01.031. Epub 2007 Feb 2.
An applicable matrix used in tissue engineering should not only have suitable mechanical properties, porous structures and biocompatibility that facilitate the adhesion, growth and proliferation of tissue cells, but also have the ability to release bioactive factors to provide a more conducive and inductive environment for tissue growth. Because of the harsh preparation conditions and deficiency of mechanical properties, it is still difficult for fibroin and collagen matrices to possess these multifunctional properties. In this research, we successfully prepared fibroin/collagen hybrid scaffolds containing heparin that possess multifunctional properties under mild conditions. These scaffolds maintain outstanding mechanical properties and porous structures of fibroin-based scaffolds. Furthermore, the scaffolds keep the bioactivity of collagen, becoming delivering systems that release heparin slowly to make the scaffolds blood compatible. Compared with fibroin/collagen scaffolds, the scaffolds containing heparin further facilitate the growth of HepG2 cells since a more complex, dynamic environment was formed to promote the cell growth. Considering the mild aqueous preparation environment without crosslinking reaction, besides promoting the progress in blood contacting tissue engineering, our research has also opened a door to prepare various multifunctional fibroin/collagen hybrid matrices that combine the advantages of fibroin and collagen.
组织工程中使用的适用基质不仅应具有合适的机械性能、多孔结构和生物相容性，以促进组织细胞的黏附、生长和增殖，还应具有释放生物活性因子的能力，为组织生长提供更有利和诱导性的环境。由于丝素蛋白和胶原蛋白基质的制备条件苛刻且机械性能不足，它们仍难以具备这些多功能特性。在本研究中，我们成功制备了含肝素的丝素蛋白/胶原蛋白混合支架，其在温和条件下具有多功能特性。这些支架保持了基于丝素蛋白的支架的出色机械性能和多孔结构。此外，支架保留了胶原蛋白的生物活性，成为缓慢释放肝素以使支架具有血液相容性的递送系统。与丝素蛋白/胶原蛋白支架相比，含肝素的支架进一步促进了HepG2细胞的生长，因为形成了更复杂、动态的环境来促进细胞生长。考虑到温和的水性制备环境且无需交联反应，除了推动血液接触组织工程的进展外，我们的研究还为制备结合丝素蛋白和胶原蛋白优势的各种多功能丝素蛋白/胶原蛋白混合基质打开了一扇门。
