Monti Daria Maria, Montesano Gesualdi Nicola, Matousek Josef, Esposito Franca, D'Alessio Giuseppe
Department of Structural and Functional Biology, University of Naples Federico II, Complesso Universitario M.S.Angelo, via Cinthia, 80126, Naples, Italy.
FEBS Lett. 2007 Mar 6;581(5):930-4. doi: 10.1016/j.febslet.2007.01.072. Epub 2007 Feb 6.
The hypothesis that the cytosolic RNase inhibitor (cRI) has a role in the protection of the cellular redox homeostasis was investigated testing the effects of oxidants and anti-oxidants on normal, primary endothelial HUVE cells, and malignant HeLa cells, before and after their engineering into cRI-deprived cells. We found that cRI plays an important, possibly a key, physiological role in the protection of cells from redox stress, as demonstrated by decreased GSH levels as well as increased oxidant-induced DNA damage in cRI deprived cells.
通过检测氧化剂和抗氧化剂对正常原代内皮HUVE细胞和恶性HeLa细胞在改造为cRI缺失细胞前后的影响，研究了胞质核糖核酸酶抑制剂（cRI）在保护细胞氧化还原稳态中发挥作用的假说。我们发现，cRI在保护细胞免受氧化还原应激方面发挥着重要的，甚至可能是关键的生理作用，这体现在cRI缺失细胞中谷胱甘肽（GSH）水平降低以及氧化剂诱导的DNA损伤增加。
