含半胱天冬酶募集结构域的凋亡抑制因子（ARC）抑制肌源性分化。
Apoptosis repressor with caspase recruitment domain (ARC) inhibits myogenic differentiation.
作者信息
Hunter Arwen L, Zhang Jingchun, Chen Shirley C, Si Xiaoning, Wong Brian, Ekhterae Daryoush, Luo Honglin, Granville David J
机构信息
James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, Department of Pathology and Laboratory Medicine, St. Paul's Hospital, University of British Columbia, 1081 Burrard Street, Vancouver, BC, Canada.
出版信息
FEBS Lett. 2007 Mar 6;581(5):879-84. doi: 10.1016/j.febslet.2007.01.050. Epub 2007 Feb 2.
Apoptosis repressor with caspase recruitment domain (ARC), an anti-apoptotic protein, is highly expressed in differentiated heart and skeletal muscle. Apoptosis and differentiation share numerous common pathways; therefore, we examined the impact of ARC on H9c2-myoblast differentiation. We demonstrate that ARC expression levels increase and stabilize upon differentiation. ARC-overexpression in pre-differentiated H9c2-cells suppresses differentiation; indicated by increased myotube formation, nuclear fusion and expression of the differentiation markers myogenin and troponin-T. ARC-overexpression inhibited myoblast differentiation associated caspase-3 activation, suggesting ARC inhibits myogenic differentiation through caspase inhibition. In summary, we show a novel role for ARC in the regulation of muscle differentiation.
含半胱天冬酶募集结构域的凋亡抑制蛋白（ARC）是一种抗凋亡蛋白，在分化的心脏和骨骼肌中高表达。细胞凋亡和分化有许多共同途径；因此，我们研究了ARC对H9c2成肌细胞分化的影响。我们证明，分化时ARC表达水平升高并稳定。预分化的H9c2细胞中ARC过表达会抑制分化；肌管形成增加、核融合以及分化标志物肌细胞生成素和肌钙蛋白-T的表达表明了这一点。ARC过表达抑制了成肌细胞分化相关的半胱天冬酶-3激活，提示ARC通过抑制半胱天冬酶来抑制肌源性分化。总之，我们揭示了ARC在肌肉分化调节中的新作用。
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