Bezerra José Antonio Ferreira, Campos Antonio Carlos L, Vasconcelos Paulo Roberto Leitão de, Nicareta Jean Ricardo, Ribeiro Elaine Rossi, Sebastião Ana Paula Martins, Urdiales Akihito Inca A, Moreira Marlus, Borges Alessandra Miguel
Universidade Federal do Maranhao.
Acta Cir Bras. 2006;21 Suppl 3:16-25. doi: 10.1590/s0102-86502006000900004.
Investigation of new substances with therapeutic effects have been done trying to isolate, extract or purify new compounds of vegetable origin. The Passiflora edulis (maracujá) species from the Plassifloracia family, originated from the tropical and subtropical regions of the american continent, is found all over Brazil. It is commonly used as a sedative, painkiller and anti-inflammatory drug and also for the treatment of skin wounds, lesions and Erisipelae.
To evaluate the wound healing in colonic anastomosis in rats that received an hydro-alcoholic extract of Passiflora edulis peri-operatively.
40 wistar rats were used distributed into two groups of 20 rats each, named: Passiflora edulis group (GP) and control group (GC). The rats of each group were separated into two subgroups of 10 animals each and were evaluated on the 3rd and 7th postoperative days. The surgical procedure consisted of a section of the left colon, 5 cm above the peritoneal reflexion with preservation of the vascular elements. Intestinal continuity was restored by an end-to-end single layer anastomosis. The Passiflora edulis group received an intraperitoneal application of the hydro-alcoholic extract in the dosage of 250 mg/kg. The control-group received one intraperitoneal dose of a saline with the same volume of the GP. The parameters evaluated were: macroscopic aspects of the wall and abdominal cavity, perianastomotic (adherences), bursting pressure, inflammatory tissue reaction on the anastomotic wound.
The macroscopic aspects did not differ between the groups. No rupture in the anastomotic wound was seen in any rat. Regarding the bursting pressure, it was noticed that the average pressure was significantly higher in the subgroup that received the Passiflora edulis extract on the 3rd day (P3) (42.6 +/- 17.8 mmHg vs. 25.4 +/- 14.1 mmHg, p=0.028), as compared to the control sub-group (C3). However, on the 7th day, bursting pressure was similar in both groups (p=0.447). Rats from the C7 sub-group had a mean bursting pressure of 203.0 +/- 50.0 mmHg vs. 187.3 +/- 39.5 mmHg in the C7 sub-group. In the histologic analysis the polymorphic nuclear cells were more frequent in the C3 group, with significant differences (p=0.034). The monomorphic nuclear cells (MMN) and the fibroblastic proliferation were more frequent in the P3 sub-group with a significant difference, p=0.02 to MMN, and p=0.001 to the fibroblastic proliferation. On the 7th day there was a significant difference in all histologic criteria stained by hematoxin-eosin and Masson Trichomic (p<0.05) in the sub-group that received the Passiflora edulis extracts.
The peri-operative administration of the hydro-alcoholic extract of Passiflora edulis has a positive influence on the healing of colonic anastomosis in rats.
为了分离、提取或纯化新的植物源化合物，人们一直在对具有治疗作用的新物质进行研究。西番莲科的西番莲（百香果）物种原产于美洲大陆的热带和亚热带地区，在巴西各地均有发现。它通常用作镇静剂、止痛药和抗炎药，还用于治疗皮肤伤口、损伤和丹毒。
评估在围手术期接受西番莲水醇提取物的大鼠结肠吻合口的愈合情况。
使用40只Wistar大鼠，分为两组，每组20只大鼠，分别命名为：西番莲组（GP）和对照组（GC）。每组大鼠再分为两个亚组，每组10只动物，并在术后第3天和第7天进行评估。手术过程包括在腹膜反折上方5 cm处切断左结肠，保留血管成分。通过端端单层吻合恢复肠道连续性。西番莲组腹腔注射剂量为250 mg/kg的水醇提取物。对照组腹腔注射与西番莲组相同体积的生理盐水。评估的参数包括：肠壁和腹腔的宏观表现、吻合口周围（粘连情况）、破裂压力、吻合口伤口的炎症组织反应。
两组的宏观表现无差异。在任何大鼠中均未观察到吻合口伤口破裂。关于破裂压力，注意到在术后第3天接受西番莲提取物的亚组（P3）的平均压力显著高于对照组亚组（C3）（42.6±17.8 mmHg对25.4±14.1 mmHg，p = 0.028）。然而，在第7天，两组的破裂压力相似（p = 0.447）。C7亚组大鼠的平均破裂压力为203.0±50.0 mmHg，而P7亚组为187.3±39.5 mmHg。在组织学分析中，C3组的多形核细胞更常见，差异有统计学意义（p = 0.034）。P3亚组的单核细胞（MMN）和成纤维细胞增殖更常见，差异有统计学意义，MMN的p = 0.02，成纤维细胞增殖的p = 0.001。在第7天，接受西番莲提取物的亚组在苏木精-伊红和马松三色染色的所有组织学标准上均有显著差异（p<0.05）。
围手术期给予西番莲水醇提取物对大鼠结肠吻合口的愈合有积极影响。
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