Dringen R, Hamprecht B
Physiologisch-chemisches Institut der Universität, Tübingen, F.R.G.
J Neurochem. 1992 Feb;58(2):511-7. doi: 10.1111/j.1471-4159.1992.tb09750.x.
The glycogen content of astroglia-rich primary cultures derived from the brains of newborn rats depends on the concentration of glucose in the culture medium. After administration of culture medium lacking glucose, the glycogen content decreases with a half-time of 7 min. Readdition of glucose results in replenishment of the glycogen stores within 2-3 h, but fully only if glucose is present in a concentration of at least 4 mM. Insulin, or the more potent insulin-like growth factor I, increases the content of glycogen approximately 1.7-fold, with the half-maximal effects being attained at concentrations of 10 and 0.5 nM, respectively. These results suggest that (a) glucose or a metabolite of it and (b) insulin-like growth factor I or a closely related peptide, but not insulin, are likely to be physiological regulators of the level of glycogen in astrocytes.
源自新生大鼠大脑的富含星形胶质细胞的原代培养物中的糖原含量取决于培养基中葡萄糖的浓度。给予缺乏葡萄糖的培养基后，糖原含量以7分钟的半衰期下降。重新添加葡萄糖会在2 - 3小时内使糖原储备得到补充，但只有当葡萄糖浓度至少为4 mM时才能完全补充。胰岛素或更强效的胰岛素样生长因子I可使糖原含量增加约1.7倍，分别在10 nM和0.5 nM的浓度下达到最大效应的一半。这些结果表明：（a）葡萄糖或其代谢产物以及（b）胰岛素样生长因子I或密切相关的肽，但不是胰岛素，可能是星形胶质细胞中糖原水平的生理调节因子。
