Obara Marcos Takashi, Barata José Maria Soares, da Silva Nilza Nunes, Ceretti Júnior Walter, Urbinatti Paulo Roberto, da Rosa João Aristeu, Jurberg José, Galvão Cleber
Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brasil.
Mem Inst Oswaldo Cruz. 2007 Feb;102(1):13-9. doi: 10.1590/s0074-02762007000100002.
The objective of this work was to analyze the morphological, morphometrical, and histological characteristics of eggs of four triatomine bugs species still not studied, in order to understand phylogenetic aspects and to facilitate parameters used in taxonomy, with the purpose of a specific and generic characterization of these vectors in public health. The eggs of each species had come from the collections of the Laboratory of Triatomine bugs and Culicid mosquitoes of the Faculdade de Saúde Pública - USP (Faculty of Public Health/USP). The morphologic studies were carried out through optic microscopy (OM) and scanning electronic microscopy (SEM). The eggs were measured with the help of the digital paquimeter Starrett 727. In relation to histological measures, Microtome Leica RM 2145 was used. Similarities were evidenced in the exocorial architecture of the eggs when they were studied by OM and SEM. This similarity among the eggs suggests a recent speciation, probably derived from common ancestry, representing a monophyletic group. Some structures were also detected by the histological cuts. The study has contributed to the magnification and recognition in generic and specific terms of the Triatominae subfamily. These new data will be able to subsidize a better understanding to determine roles for each vector species and to facilitate parameters to be used in taxonomy.
这项工作的目的是分析四种尚未研究的锥蝽卵的形态、形态测量和组织学特征,以了解系统发育方面的情况,并促进分类学中使用的参数,从而对这些公共卫生媒介进行属和种的特异性表征。每个物种的卵均来自圣保罗大学公共卫生学院(Faculdade de Saúde Pública - USP)锥蝽和库蚊实验室的收藏。形态学研究通过光学显微镜(OM)和扫描电子显微镜(SEM)进行。借助Starrett 727数字测微计对卵进行测量。对于组织学测量,使用了徕卡RM 2145切片机。通过OM和SEM研究卵时,在卵的卵壳结构中发现了相似之处。卵之间的这种相似性表明最近发生了物种形成,可能源自共同祖先,代表一个单系类群。通过组织切片还检测到了一些结构。该研究有助于在属和种的层面上对锥蝽亚科进行放大和识别。这些新数据将有助于更好地理解每个媒介物种的作用,并促进分类学中使用的参数。