大鼠中枢神经系统培养物中一种独特类型的GD3 +扁平星形胶质细胞。
A distinct type of GD3+, flat astrocyte in rat CNS cultures.
作者信息
Vaysse P J, Goldman J E
机构信息
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
出版信息
J Neurosci. 1992 Jan;12(1):330-7. doi: 10.1523/JNEUROSCI.12-01-00330.1992.
We have identified what is apparently a distinct type of astrocyte in primary cultures from several regions of the neonatal rat CNS. These cells express GD3 ganglioside for long periods in vitro, and are GFAP+, but do not express the oligodendrocyte antigens O4 or galactocerebroside (GC). The majority, but not all, are A2B5+. The cells grow in a flat, highly spread morphology with many thin cytoplasmic processes. Gene transfer with a replication-deficient retrovirus combined with immunostaining for astro- and oligodendroglial markers (antibodies to GFAP, GD3 ganglioside, GC, and the A2B5 and O4 antibodies) demonstrated that in the neonatal rat CNS cultures these cells are clonally separate from oligodendrocytes and from the majority of (GD3-) astrocytes. The clonal analysis suggests a distinct progenitor cell and a distinct developmental sequence for these astrocytes.
我们已经在新生大鼠中枢神经系统几个区域的原代培养物中鉴定出一种明显独特的星形胶质细胞类型。这些细胞在体外长时间表达GD3神经节苷脂，且GFAP呈阳性，但不表达少突胶质细胞抗原O4或半乳糖脑苷脂（GC）。大多数细胞（但并非全部）A2B5呈阳性。这些细胞以扁平、高度伸展的形态生长，具有许多细长的细胞质突起。利用复制缺陷型逆转录病毒进行基因转移，并结合对星形胶质细胞和少突胶质细胞标志物（抗GFAP、GD3神经节苷脂、GC以及A2B5和O4抗体）的免疫染色表明，在新生大鼠中枢神经系统培养物中，这些细胞与少突胶质细胞以及大多数（GD3阴性）星形胶质细胞在克隆上是分开的。克隆分析表明这些星形胶质细胞有独特的祖细胞和独特的发育序列。
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