Vaysse P J, Goldman J E
Department of Pathology, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Neuron. 1990 Sep;5(3):227-35. doi: 10.1016/0896-6273(90)90160-h.
Retrovirus-mediated gene transfer combined with triple immunostaining for astro- and oligodendroglial markers (antibodies to glial fibrillary acidic protein, GD3 ganglioside, and galactocerebroside, and the O4 antibody) was used to study clonal aspects of glial lineage in primary cultures of the neonatal rat striatum. We found two major clonal populations: astrocyte clones containing GFAP+, but GD3-, O4-, and GC- cells, and oligodendrocyte clones containing cells expressing various combinations of GD3, O4, and GC, with rare GFAP+ cells. These results indicate that astrocytes and oligodendrocytes belong to separate lineages in forebrain postnatal development.
逆转录病毒介导的基因转移结合针对星形胶质细胞和少突胶质细胞标志物的三重免疫染色（抗胶质纤维酸性蛋白、GD3神经节苷脂、半乳糖脑苷脂抗体以及O4抗体），用于研究新生大鼠纹状体原代培养物中神经胶质谱系的克隆情况。我们发现了两个主要的克隆群体：星形胶质细胞克隆，包含GFAP+但GD3-、O4-和GC-细胞；少突胶质细胞克隆，包含表达GD3、O4和GC各种组合的细胞，GFAP+细胞罕见。这些结果表明，在出生后脑发育过程中，星形胶质细胞和少突胶质细胞属于不同的谱系。
