Liao Min, Xie Xiao-Mei
School of Environmental and Resources Sciences, Zhejiang University, Hangzhou 310029, China.
J Environ Sci (China). 2006;18(3):510-3.
The degradative characteristics of phenanthrene, microbial biomass carbon, plate counts of heterotrophic bacteria and most probable number (MPN) of phenanthrene degraders in non-rhizosphere or rhizosphere soils with uninoculating or inoculating phenanthrene degraders were measured. At the initial concentration of 20 mg phenanthrene/kg soil, the half-lives of phenanthrene in uninoculated non-rhizosphere soil, uninoculated rhizosphere soil, inoculated non-rhizosphere soil, and inoculated rhizosphere soil were measured to be 81.5, 47.8, 15.1 and 6.4 d, respectively, and corresponding kinetic data fitted first-order kinetics. The highest degradation rate of phenanthrene was observed in inoculated rhizosphere soil. The degradative characteristics of phenanthrene were closely related to the effects of vegetation on soil microbial process. Vegetation could enhance the magnitude of rhizosphere microbial communities, microbial biomass content, and heterotrophic bacterial community, but barely influence those community components responsible for phenanthrene degradation. Results suggested that combination of vegetation and inoculation with degrading microorganisms of target organic contaminants was a better pathway to enhance degradation of the organic contaminants in soil.
测定了在未接种或接种菲降解菌的非根际或根际土壤中，菲的降解特性、微生物生物量碳、异养细菌平板计数以及菲降解菌的最可能数（MPN）。在菲初始浓度为20mg/kg土壤时，未接种的非根际土壤、未接种的根际土壤、接种的非根际土壤和接种的根际土壤中菲的半衰期分别测定为81.5、47.8、15.1和6.4天，相应的动力学数据符合一级动力学。在接种的根际土壤中观察到菲的最高降解率。菲的降解特性与植被对土壤微生物过程的影响密切相关。植被可以增强根际微生物群落、微生物生物量含量和异养细菌群落的规模，但对负责菲降解的那些群落组成部分影响很小。结果表明，植被与接种目标有机污染物降解微生物相结合是增强土壤中有机污染物降解的更好途径。
