Wang Dan-qing, Peng Zhi-lan, Niu Xiao-yu, Li Da-ke
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Jan;38(1):6-8, 48.
To test of the effect of vector-based RNA interference (RNAi) technique on inhibiting HPV16E7 gene in CaSki cells of cervical cancer.
The HPV16E7-specific siRNA expression vectors P1, P2 and P3 were constructed and transfected into CaSki cells by liposome. The expression of HPV16E7 mRNA and protein were detected by real-time RT-PCR and Western blot.
The expression of HPV16E7 mRNA and protein decreased with the transfection of P1, P2 and P3. Vector P1 had the strongest inhibition effect, with an inhibition rates of 92.86% and 81.0% for the expression of HPV16E7 mRNA and protein respectively three weeks after transfection.
The expression of E7 gene in CaSki cells can be inhibited by HPV16E7-specific siRNA expression vector.
检测基于载体的RNA干扰（RNAi）技术对宫颈癌CaSki细胞中HPV16E7基因的抑制作用。
构建HPV16E7特异性siRNA表达载体P1、P2和P3，通过脂质体转染至CaSki细胞。采用实时RT-PCR和Western blot检测HPV16E7 mRNA和蛋白的表达。
P1、P2和P3转染后，HPV16E7 mRNA和蛋白的表达均下降。载体P1的抑制作用最强，转染3周后，HPV16E7 mRNA和蛋白表达的抑制率分别为92.86%和81.0%。
HPV16E7特异性siRNA表达载体可抑制CaSki细胞中E7基因的表达。
