Zhang Peng-bo, Liu Yong, Li Jie, Kang Qian-yan, Tian Ying-fang, Zhao Jian-jun, Shi Qin-dong
Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Jan;38(1):146-9.
To identify the labeled cells in the ependyma/subventricular zone (SVZ) of normal adult rats by DiI injected into the lateral ventricle.
Fifty male Sprague-Dawley rats were divided randomly into five groups (10 per group). All of the rats were injected with 10 microL of 2 g/L fluorescence dye DiI into the right lateral ventricle. The five groups of rats were sacrificed at 6 h, 12 h, 24 h, 36 h or 48 h after injection respectively. Hoechst 33258 staining was used to identify nuclei and laser confocal microscopy was used to detect the DiI-labeled cells and to measure the thickness of the tissue with DiI fluorescence in the wall of the left lateral ventricle.
After injection of the DiI into the right lateral ventricle, DiI- Hoechst 33258 double positive cells were found in the ependymal layer of the left lateral ventricular wall at 24 h and in the SVZ at 48 h as well. The thickness of the tissue with DiI fluorescence in the left ependyma/septal subventricular zone (SVZspt) and ependyma/postnatal equivalent of the ganglionic eminences (SVZge) remained unchanged at 12 h and 24 h after DiI injection. The thickness of the tissue with DiI fluorescence in the left ependyma/SVZge was significantly greater than that in the ependyma/SVZspt at all of the time points (P<0.05).
The ependyma/SVZ cells can be labeled by Dil 24-48 h after injection (10 microL of 2 g/L) into the lateral ventricle.
通过向成年正常大鼠侧脑室注射DiI来识别室管膜/室下区（SVZ）中的标记细胞。
50只雄性Sprague-Dawley大鼠随机分为五组（每组10只）。所有大鼠均向右侧脑室注射10μL 2g/L荧光染料DiI。五组大鼠分别在注射后6小时、12小时、24小时、36小时或48小时处死。采用Hoechst 33258染色鉴定细胞核，并用激光共聚焦显微镜检测DiI标记的细胞，并测量左侧脑室壁中具有DiI荧光的组织厚度。
向右侧脑室注射DiI后，在24小时时于左侧脑室壁的室管膜层发现DiI-Hoechst 33258双阳性细胞，48小时时在室下区也发现此类细胞。在注射DiI后12小时和24小时，左侧室管膜/隔区室下区（SVZspt）和室管膜/神经节隆起出生后对应区域（SVZge）中具有DiI荧光的组织厚度保持不变。在所有时间点，左侧室管膜/SVZge中具有DiI荧光的组织厚度均显著大于室管膜/SVZspt中的厚度（P<0.05）。
向侧脑室注射（10μL 2g/L）DiI后24 - 48小时，室管膜/SVZ细胞可被标记。
