Migita Kiyoshi, Miyashita Taichiro, Maeda Yumi, Nakamura Minoru, Yatsuhashi Hiroshi, Kimura Hironori, Ishibashi Hiromi, Eguchi Katsumi
Clinical Research Center and Department of General Internal Medicine, NHO Nagasaki Medical Center, Omura, Japan.
J Rheumatol. 2007 Mar;34(3):493-500.
To investigate expression of members of the Toll-like receptor (TLR) family in peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE).
We analyzed PBMC from 14 patients with SLE and 15 healthy subjects. The surface expressions of TLR2 and TLR4 and intracellular expression of TLR9 on PBMC were analyzed by flow cytometry.
Although TLR4 expressions on CD14+ monocytes were not significantly different between healthy subjects and patients with SLE, TLR2 expressions on monocytes were reduced in patients with SLE compared to healthy subjects. Intracellular TLR9 expression levels of CD19+ B lymphocytes were significantly elevated in patients with SLE. However, the TLR9 expression levels of plasmacytoid dendritic cells were not significantly different between these patients and healthy subjects.
Our results show that human peripheral blood B cells express TLR9 and that its expression is increased in patients with SLE. This upregulated expression of TLR9 in B cells may be related to the abnormal B cell hyperactivity in patients with SLE.
研究系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中Toll样受体（TLR）家族成员的表达情况。
我们分析了14例SLE患者和15名健康受试者的PBMC。采用流式细胞术分析PBMC上TLR2和TLR4的表面表达以及TLR9的细胞内表达。
尽管健康受试者与SLE患者CD14+单核细胞上的TLR4表达无显著差异，但与健康受试者相比，SLE患者单核细胞上的TLR2表达降低。SLE患者CD19+B淋巴细胞的细胞内TLR9表达水平显著升高。然而，这些患者与健康受试者之间浆细胞样树突状细胞的TLR9表达水平无显著差异。
我们的结果表明，人外周血B细胞表达TLR9，且其表达在SLE患者中增加。B细胞中TLR9的这种上调表达可能与SLE患者B细胞异常活跃有关。
