与等速电泳技术相比，采用大体积样品堆积和动态pH交界相结合的方法通过毛细管电泳对弱电解质进行在线预富集。

Combination of large volume sample stacking and dynamic pH junction for on-line preconcentration of weak electrolytes by capillary electrophoresis in comparison with isotachophoretic techniques.

作者信息

Horáková Jana, Petr Jan, Maier Vítezslav, Znaleziona Joanna, Stanová Andrea, Marák Jozef, Kaniansky Dusan, Sevcík Juraj

机构信息

Department of Analytical Chemistry, Palacký University, Trída Svobody 8, Olomouc, Czech Republic.

出版信息

J Chromatogr A. 2007 Jul 6;1155(2):193-8. doi: 10.1016/j.chroma.2007.01.075. Epub 2007 Jan 28.

DOI:10.1016/j.chroma.2007.01.075

PMID:17296203

Abstract

An on-line preconcentration capillary electrophoresis (CE) technique, which combines a large volume sample stacking with a dynamic pH junction technique, is introduced in this paper. This dynamic pH junction with co-electroosmotic migration is formed between sodium borate pH 9.5 and sodium phosphate pH 2.5 with 150 mM sodium dodecylsulfate (SDS). A full capillary based injection allows determination of weak acidic compounds at ppb concentration levels (achieved LOD for benzoic acid was 11 nmol L(-1)). The proposed preconcentration method was compared with ITP/ITP (LOD 120 nmol L(-1)), ITP/CZE (LOD 740 nmol L(-1)) and a simple CZE method (LOD 23,330 nmol L(-1)). The analytical potential of this method was assessed with juice test samples.

摘要

本文介绍了一种在线预浓缩毛细管电泳（CE）技术，该技术将大体积样品堆积与动态pH连接技术相结合。在pH 9.5的硼酸钠和pH 2.5的磷酸钠之间，加入150 mM十二烷基硫酸钠（SDS），形成具有共电渗迁移的动态pH连接。基于全毛细管的进样方式可测定ppb浓度水平的弱酸性化合物（苯甲酸的检测限为11 nmol L(-1)）。将所提出的预浓缩方法与ITP/ITP（检测限120 nmol L(-1)）、ITP/CZE（检测限740 nmol L(-1)）和简单的CZE方法（检测限23,330 nmol L(-1)）进行了比较。用果汁测试样品评估了该方法的分析潜力。