Azad A F, Sacci J B, Nelson W M, Dasch G A, Schmidtmann E T, Carl M
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):43-6. doi: 10.1073/pnas.89.1.43.
The identification of apparently fastidious microorganisms is often problematic. DNA from a rickettsia-like agent (called the ELB agent) present in cat fleas could be amplified by PCR with conserved primers derived from rickettsial 17-kDa common protein antigen and citrate synthase genes but not spotted fever group 190-kDa antigen gene. Alu I sites in both the 17-kDa and citrate synthase PCR products obtained with the rickettsia-like agent and Rickettsia typhi were different even though both agents reacted with monoclonal antibodies previously thought specific for R. typhi. The DNA sequence of a portion of the 17-kDa PCR product of the rickettsia-like agent differed significantly from all known rickettsial sequences and resembled the 17-kDa sequences of typhus more than spotted fever group rickettsiae. The rare stable transovarial maintenance of this rickettsia in cat fleas has important implications for the disease potential of cat fleas.
鉴定明显挑剔的微生物往往存在问题。猫蚤中存在的一种立克次氏体样病原体（称为ELB病原体）的DNA，可通过聚合酶链反应（PCR），用源自立克次氏体17 kDa共同蛋白抗原和柠檬酸合酶基因的保守引物进行扩增，但不能用斑点热群190 kDa抗原基因进行扩增。用立克次氏体样病原体和鼠型斑疹伤寒立克次氏体获得的17 kDa和柠檬酸合酶PCR产物中的Alu I位点不同，尽管这两种病原体都与先前认为对鼠型斑疹伤寒立克次氏体具有特异性的单克隆抗体发生反应。立克次氏体样病原体17 kDa PCR产物的一部分DNA序列与所有已知立克次氏体序列有显著差异，与斑疹伤寒的17 kDa序列的相似性超过斑点热群立克次氏体。这种立克次氏体在猫蚤中罕见的稳定经卵传递对猫蚤的致病潜力具有重要意义。
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