[应用多色荧光原位杂交技术检测膀胱尿路上皮癌]
[Detection of urothelial carcinoma of the urinary bladder by multicolor fluorescence in situ hybridization].
作者信息
Zhang Ye-Gui, Bi Xin-Gang, Han Ya-Ling, Cai Yan, Xu Xin, Wu Yu-Peng, Yang Yi-Ling, Ma Jian-Hui, Zhao Ping, Jia Xue-Mei, Wang Ming-Rong
机构信息
Department of Histology and Embryology, Anhui Medical University,Hefei, Anhui, PR China.
出版信息
Ai Zheng. 2007 Feb;26(2):189-93.
BACKGROUND & OBJECTIVE: Urothelial carcinoma of the urinary bladder is a common malignant neoplasm of the genitourinary system. This study was to analyze chromosome aberrations in urothelial carcinoma of the urinary bladder in Chinese, and evaluate the possibility and validity of multicolor fluorescence in situ hybridization (M-FISH) in detecting urothelial carcinoma of the urinary bladder.
METHODS
The probes of chromosome 3, 7, 17 centromeres and 9p21 region were labeled by random primer method. M-FISH was performed on interphase nuclei of 57 samples of urothelial carcinoma to analyze the correlations of chromosome aberration to diagnosis and pathology of urothelial carcinoma.
RESULTS
The rate of aneuploidy was 47.4% for chromosome 3, 50.9% for chromosome 7, 56.1% for chromosome 17, and 59.6% for chromosome 9p21 in urothelial carcinoma. All the aberrations had no correlations to tumor stage. The aberrations of chromosomes 3, 7, 17 were significantly correlated to pathologic grade (P<0.01). As using the 4 chromosome probes in combination, the sensitivity for detecting urothelial carcinoma of the urinary bladder was 54.4%.
CONCLUSION
M-FISH may be helpful for detecting urothelial carcinoma of the urinary bladder.
背景与目的
膀胱尿路上皮癌是泌尿生殖系统常见的恶性肿瘤。本研究旨在分析中国膀胱尿路上皮癌的染色体畸变情况，并评估多色荧光原位杂交（M-FISH）检测膀胱尿路上皮癌的可能性及有效性。
方法
采用随机引物法标记3号、7号、17号染色体着丝粒及9p21区域的探针。对57例膀胱尿路上皮癌样本的间期核进行M-FISH，分析染色体畸变与膀胱尿路上皮癌诊断及病理的相关性。
结果
膀胱尿路上皮癌中，3号染色体非整倍体率为47.4%，7号染色体为50.9%，17号染色体为56.1%，9p21区域为59.6%。所有畸变均与肿瘤分期无关。3号、7号、17号染色体畸变与病理分级显著相关（P<0.01）。联合使用4种染色体探针时，检测膀胱尿路上皮癌的灵敏度为54.4%。
结论
M-FISH可能有助于检测膀胱尿路上皮癌。
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