Wenzel Pamela L, Leone Gustavo
Genesis. 2007 Mar;45(3):129-34. doi: 10.1002/dvg.20276.
An increasing number of genes known to be critical for cell cycle control, differentiation, and tumor suppression have been found to impact development of the placenta. To elucidate how these genes contribute to development of embryonic and extra-embryonic lineages, we generated a transgenic mouse in which the Cre transgene is driven by placenta-specific regulatory sequences from the human CYP19 gene. Using ROSA26 conditional reporter mice, we could detect expression of the CYP19-Cre transgene throughout the extra-embryonic ectoderm and in the ectoplacental cone at embryonic day 6.5 (E6.5). By E11.5, recombination of LoxP reporter sites was detected in all derivatives of trophoblast stem cells, including spongiotrophoblast, giant cells, and labyrinth trophoblasts. We conclude that the CYP19-Cre transgenic mouse developed here can be used in combination with conditional alleles to distinguish between embryonic and extra-embryonic gene function, and to begin to map the period of time when gene function is critical during development.
越来越多已知对细胞周期调控、分化和肿瘤抑制至关重要的基因被发现会影响胎盘的发育。为了阐明这些基因如何促进胚胎和胚外谱系的发育，我们构建了一种转基因小鼠，其中Cre转基因由人CYP19基因的胎盘特异性调控序列驱动。利用ROSA26条件性报告基因小鼠，我们能够在胚胎第6.5天（E6.5）检测到CYP19-Cre转基因在整个胚外外胚层和外胎盘锥中的表达。到E11.5时，在滋养层干细胞的所有衍生物中都检测到了LoxP报告位点的重组，包括海绵滋养层、巨细胞和迷路滋养层。我们得出结论，这里构建的CYP19-Cre转基因小鼠可与条件性等位基因结合使用，以区分胚胎和胚外基因功能，并开始确定基因功能在发育过程中至关重要的时间段。
