Wilson T, Lewis M J, Cha K L, Gold B
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805.
Cancer Lett. 1992 Jan 10;61(2):129-34. doi: 10.1016/0304-3835(92)90170-z.
Ellagic acid (EA) is an inhibitor of the in vitro mutagenicity of N-nitrosodimethylamine (NDMA) in Salmonella typhimurium strain TA100 using pyrazole-induced rat liver 9000 x g supernatant (S-9). In order to understand this activity, the effect of EA on the metabolic hydroxylation of 4-nitrophenol, a substrate, as is NDMA, for cytochrome P-450IIE1 was studied using pyrazole induced rat S-9 and microsomal protein. It is shown that EA has an inhibitory effect on 4-nitrophenol hydroxylase with both enzyme preparations. This effect on cytochrome P-450IIE1 may be responsible, at least in part, for the inhibition of NDMA mutagenicity by EA.
鞣花酸（EA）是一种在使用吡唑诱导的大鼠肝脏9000×g上清液（S-9）的情况下，对鼠伤寒沙门氏菌TA100菌株中N-亚硝基二甲胺（NDMA）的体外诱变性具有抑制作用的物质。为了了解这种活性，使用吡唑诱导的大鼠S-9和微粒体蛋白，研究了EA对4-硝基苯酚（一种与NDMA一样作为细胞色素P-450IIE1底物）代谢羟基化的影响。结果表明，EA对两种酶制剂的4-硝基苯酚羟化酶均有抑制作用。这种对细胞色素P-450IIE1的作用可能至少部分地是EA抑制NDMA诱变性的原因。
